Impact of human visceral and glutealfemoral adipose tissue transplant on glycemic control in a mouse model of diet-induced obesity.

Impact of human visceral and glutealfemoral adipose tissue transplant on glycemic control in a mouse model of diet-induced obesity. Am J Physiol Endocrinol Metab. 2020 Jun 30;: Authors: Tsiloulis T, Raajendiran A, Keenan SN, Ooi G, Taylor RA, Burton P, Watt MJ Abstract Regional distribution of adipose tissue is an important factor in conferring cardiometabolic risk and obesity-related morbidity. We tested thehypothesis that human visceral adipose tissue (VAT) impairs glucose homeostasis, whereas subcutaneous glutealfemoral adipose tissue (GFAT)protects against the development of impaired glucose homeostasis in mice.VAT and GFAT were collected from patients undergoing bariatric surgery and grafted onto the epididymal adipose tissue of weight and age-matched severe combined immunodeficient mice. SHAM mice underwent surgery without transplant of tissue. Mice were fed a high-fat diet after xenograft. Energy homeostasis, glucose metabolism and insulin sensitivity were assessed six weeks later.Xenograftof human adipose tissues was successful as determined by histology, immunohistochemical evaluation of collagen deposition and angiogenesis, and maintenance of lipolytic function. Adipose tissue transplant did not affect energy expenditure, food intake, whole-body substrate partitioning, or plasma free fatty acid, triglyceride and insulin levels. Fasting blood glucose was significantly reduced in GFAT and VAT compared with SHAM, whereas gluco...
Source: American Journal of Physiology. Endocrinology and Metabolism - Category: Physiology Authors: Tags: Am J Physiol Endocrinol Metab Source Type: research