Development of a new GC –MS/MS method for the determination of metformin in human hair

In this study the authors have developed a GC/MS–MS method for the detection and the quantification of metformin in human hair and have applied it to patients under treatment. Measured concentrations range from 0.3 to 3.8 ng/mg. Me tformin appears to be poorly incorporated into hair, given a daily dose of 1 to 3 g. There is probably a correlation between dose and concentration in dark hair. AbstractDiabetes mellitus is one of the most important public health challenges. Metformin (1,1 ‐dimethylbiguanide) represents the “gold standard” for the treatment of diabetes mellitus type 2. Despite its important role in reducing mortality and morbidity in the diabetic population, metformin is associated with an increased risk of stroke. To document exposure to a drug, hair is conside red to be the specimen of choice to complement blood and urine, since it provides historical detail of a subject's chronic exposure to drug(s). Measuring hair concentration of metformin can be important for forensic toxicologists investigating criminal poisoning or Munchausen's syndrome by proxy. In clinical toxicology, drug monitoring using hair to document metformin observance has not yet been described. To document the interest of hair analysis for metformin, the authors have developed and validated a method using a gas‐chromatography tandem mass spectrometry system and applied it to auth entic hair obtained from 9 diabetic patients under daily treatment. The validation procedure demonstrated ...
Source: Drug Testing and Analysis - Category: Drugs & Pharmacology Authors: Tags: SHORT COMMUNICATION Source Type: research