GSE147694 Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease

Contributors : Elisa Giacomelli ; Viviana Meraviglia ; Giulia Campostrini ; Amy Cochrane ; Xu Cao ; Ruben van Helden ; Ana Krotenberg Garcia ; Maria Mircea ; Sarantos Kostidis ; Richard P Davis ; Berend J van Meer ; Carolina R Jost ; Abraham J Koster ; Hailiang Mei ; David G M íguez ; Aat A Mulder ; Mario Ledesma-Terrón ; Giulio Pompilio ; Luca Sala ; Daniela Salvatori ; Roderick Slieker ; Elena Sommariva ; Antoine de Vries ; Martin Giera ; Stefan Semrau ; Leon Tertoolen ; Valeria V Orlova ; Milena Bellin ; Christine L MummerySeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCardiomyocytes (CMs) from human induced pluripotent stem cells (hiPSCs) are functionally immature, but this is improved by incorporation into engineered tissues or forced contraction. Here, we showed that tri-cellular combinations of hiPSC-derived CMs, cardiac fibroblasts (CFs), and cardiac endothelial cells also enhance maturation in easily constructed, scaffold-free, three-dimensional microtissues (MTs). hiPSC-CMs in MTs with CFs showed improved sarcomeric structures with T-tubules, enhanced contractility, and mitochondrial respiration and were electrophysiologically more mature than MTs without CFs. Interactions mediating maturation included coupling between hiPSC-CMs and CFs through connexin 43 (CX43) gap junctions and increased intracellular cyclic AMP (cAMP). Scaled production of thousands of hiPSC-MTs was highly reproducible across lines a...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research