MsrA efflux pump inhibitory activity of Piper cubeba L.f. and its phytoconstituents against Staphylococcus aureus RN4220.

MsrA efflux pump inhibitory activity of Piper cubeba L.f. and its phytoconstituents against Staphylococcus aureus RN4220. Chem Biodivers. 2020 May 24;: Authors: Ahirrao P, Tambat R, Chandal N, Mahey N, Kamboj A, Jain UK, Singh IP, Jachak SM, Nandanwar HS Abstract <p>Methionine sulfoxide reductase A (MsrA), an efflux pump belonging to ATP-binding cassette (<st1:stockticker w:st="on">ABC)</st1:stockticker> transporter family that conferred resistance to macrolides, was detected in Methicillin-Resistant <em>Staphylococcus aureus</em> (<st1:stockticker w:st="on">MRSA</st1:stockticker>) RN4220 strain. Herein we report isolation of phytoconstituents from<em> Piper cubeba</em> fruit methanol extract and investigated their efflux pump inhibitory potential against MsrA pump. Four isolated compounds viz. pellitorine, sesamin, piperic acid and tetrahydropiperine when studied in combination with erythromycin in <em>S. aureus </em>RN4220, exhibited 2-8 fold reduction in Minimum inhibitory concentration (MIC) of erythromycin. Pellitorine and sesamin decreased MIC of erythromycin by 8-fold. The real-time fluorometry-based efflux and accumulation studies of ethidium bromide (EtBr) on <em>S. aureus </em>RN4220 in the presence of these compounds showed reduced efflux and enhanced uptake, thus indicating inhibition of the efflux pump. Pellitorine showed significant post-antib...
Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Tags: Chem Biodivers Source Type: research