A novel fluorescent hydroxyapatite based on iron quantum cluster template to enhance osteogenic differentiation.

A novel fluorescent hydroxyapatite based on iron quantum cluster template to enhance osteogenic differentiation. Mater Sci Eng C Mater Biol Appl. 2020 Jun;111:110775 Authors: Hashemi N, Vaezi Z, Khanmohammadi S, Naderi Sohi A, Masoumi S, Hruschka V, Wolbank S, Redl H, Marolt Presen D, Naderi-Manesh H Abstract Template-mediated self-assembly synthesis has produced a diverse range of biomimetic materials with unique physicochemical properties. Here, we fabricated novel fluorescent three-dimensional (3-D) hydroxyapatite (HAP) nanorod-assembled microspheres using iron quantum cluster (FeQC) as a hybrid template, containing three organic components: hemoglobin chains, piperidine, and iron clusters. The material characterization indicated that the synthesized HAP possessed a uniform rod-like morphology, ordered 3-D architecture, high crystallinity, self-activated fluorescence, and remarkable photostability. Our study proposed that this FeQC template is a promising regulating agent to fabricate fluorescent self-assembled HAP microspheres with a controlled morphology. The effect of HAP on stem cell fate and their osteogenic differentiation was investigated by culturing human bone marrow-derived mesenchymal stromal/stem cells (BMSCs) with HAP microspheres. Significant increases in collagen matrix production and gene expression of osteogenic markers, including osteocalcin (OCN), Runt-related transcription factor 2 (Runx2), bone sialoprotein (B...
Source: Appl Human Sci - Category: Physiology Authors: Tags: Mater Sci Eng C Mater Biol Appl Source Type: research