Cancers, Vol. 12, Pages 780: Aptamer-Equipped Protamine Nanomedicine for Precision Lymphoma Therapy

Cancers, Vol. 12, Pages 780: Aptamer-Equipped Protamine Nanomedicine for Precision Lymphoma Therapy Cancers doi: 10.3390/cancers12040780 Authors: Zihua Zeng Ching-Hsuan Tung Youli Zu Anaplastic large cell lymphoma (ALCL) is the most common T-cell lymphoma in children. ALCL cells characteristically express surface CD30 molecules and carry the pathogenic ALK oncogene, both of which are diagnostic biomarkers and are also potential therapeutic targets. For precision therapy, we report herein a protamine nanomedicine incorporated with oligonucleotide aptamers to selectively target lymphoma cells, a dsDNA/drug payload to efficiently kill targeted cells, and an siRNA to specifically silence ALK oncogenes. The aptamer-equipped protamine nanomedicine was simply fabricated through a non-covalent charge-force reaction. The products had uniform structure morphology under an electron microscope and a peak diameter of 103 nm by dynamic light scattering measurement. Additionally, flow cytometry analysis demonstrated that under CD30 aptamer guidance, the protamine nanomedicine specifically bound to lymphoma cells, but did not react to off-target cells in control experiments. Moreover, specific cell targeting and intracellular delivery of the nanomedicine were also validated by electron and confocal microscopy. Finally, functional studies demonstrated that, through combined cell-selective chemotherapy using a drug payload and oncogene-specific gene therapy using an siRNA, the prot...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research