Molecules, Vol. 25, Pages 964: Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis ‐ Inducing Effects on Human Leukemic Cells

Molecules, Vol. 25, Pages 964: Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis‐ Inducing Effects on Human Leukemic Cells Molecules doi: 10.3390/molecules25040964 Authors: Koutova Kulhava Havelek Majorosova Královec Habartova Hošťálková Opletal Cahlikova Řezáčová Bersavine is the new bisbenzylisoquinoline alkaloid isolated from the Berberis vulgaris L.(Berberidaceae) plant. The results of cytotoxicity screening 48 h post‐treatment showed thatbersavine considerably inhibits the proliferation and viability of leukemic (Jurkat, MOLT‐4), colon(HT‐29), cervix (HeLa) and breast (MCF‐7) cancer cells with IC50 values ranging from 8.1 to 11 μM.The viability and proliferation of leukemic Jurkat and MOLT‐4 cells were decreased after bersavinetreatment in a time‐ and dose‐dependent manner. Bersavine manifested concentration‐dependentantiproliferative activity in human lung, breast, ovarian and hepatocellular carcinoma cell linesusing a xCELLigence assay. Significantly higher percentages of MOLT‐4 cells exposed to bersavineat 20 μM for 24 h were arrested in the G1 phase of the cell cycle using the flow cytometry method.The higher percentage of apoptotic cells was measured after 24 h of bersavine treatment. Theupregulation of p53 phosphorylated on Ser392 was detected during the progression of MOLT‐4 cellapoptosis. Mechanistically, bersavine‐induced a...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research