Accuracy of the ELITe MGB® assays for the detection of carbapenemases, CTX-M, Staphylococcus aureus and mecA/C genes directly from respiratory samples

This study aimed to determine whether molecular assays could detect genes encoding carbapenemases, ESBL and MRSA, directly from respiratory samples, so as to expedite appropriate therapy and infection control for patients with BLRTI.MethodsThe CRE, ESBL and MRSA/SA ELITe MGB® assays were performed directly on 354 respiratory specimens sampled from 318 patients admitted with BLRTI. Molecular results were compared to routine culture-based diagnostics results.ResultsPositive (PPV) and negative (NPV) predictive values of the CRE ELITe MGB® kit were 75.9% [IC 95%: 60.3-86.7] and 100%, respectively. PPV and NPV of the ESBL ELITe MGB® kit were 80.8% [IC 95%: 63.6-91] and 99.1% [IC 95%: 96.6-99.8], respectively. PPV and NPV predictive values of the MRSA/SA ELITe MGB® kit were 91.7% [IC 95%: 73.7-97.7]/100% and 98.3% [IC 95%: 89.8-99.3]/96.8% [IC 95%: 81.6-99.5], respectively.DiscussionValidity assessment of molecular assays detecting the main antibiotic resistance genes directly from respiratory samples showed a high accuracy when compared to culture-based results. Molecular assays detecting the main carbapenemase, ESBL, S. aureus and methicillin resistance encoding genes provide an interesting tool with potential to expedite optimization of antibiotic therapy and infection control practices in patients with BLRTI.
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research