The effect of magnetic field exposure on differentiation of magnetite nanoparticle-loaded adipose-derived stem cells

Publication date: April 2020Source: Materials Science and Engineering: C, Volume 109Author(s): Luminita Labusca, Dumitru-Daniel Herea, Camelia-Mihaela Danceanu, Anca Emanuela Minuti, Cristina Stavila, Marian Grigoras, Daniel Gherca, George Stoian, Gabriel Ababei, Horia Chiriac, Nicoleta LupuAbstractMagnetic nanoparticles (MNPs) are versatile tools for various applications in biotechnology and nanomedicine. MNPs-mediated cell tracking, targeting and imaging are increasingly studied for regenerative medicine applications in cell therapy and tissue engineering. Mechanical stimulation influences mesenchymal stem cell differentiation. Here we show that MNPs-mediated magneto-mechanical stimulation of human primary adipose derived stem cells (ADSCs) exposed to variable magnetic field (MF) influences their adipogenic and osteogenic differentiation. ADSCs loaded with biocompatible magnetite nanoparticles of 6.6 nm, and with an average load of 21 picograms iron/cell were exposed to variable low intensity (0.5 mT - LMF) and higher intensity magnetic fields (14.7 and 21.6 mT - HMF). Type, duration, intensity and frequency of MF differently affect differentiation. Short time (2 days) intermittent exposure to LMF increases adipogenesis while longer (7 days) intermittent as well as continuous exposure favors osteogenesis. HMF (21.6 mT) short time intermittent exposure favors osteogenesis. Different exposure protocols can be used to increase differentiation dependently on expected resu...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research