Antifungal and anti-biofilm activity of designed derivatives from Kyotorphin

This study assessed the antimicrobial and antibiofilm activity of these peptides. The antifungal activity of kyotorphins was determined in representative strains of Candida species, including C. albicans ATCC 10231, C. krusei ATCC 6258, and six clinical isolates—C. dubliniensis 19-S, C. glabrata 217-S, C. lusitaniae 14-S, C. novergensis 51-S, C. parapsilosis 63, and C. tropicalis 140-S—obtained from the oral cavity of HIV-positive patients. The peptides were synthesized by standard solution or solid-phase synthesis, purified by RP-HPLC (purity>95%), and characterized by nuclear magnetic resonance. The results of the broth microdilution assay and scanning electron microscopy showed that IbKTP-NH2 presented significant antifungal activity against Candida strains and antibiofilm activity against the clinical isolates. The absence of toxic activity and survival after infection was assessed after injecting the peptide in larvae of Galleria mellonella as experimental infection model. Furthermore, IbKTP-NH2 had strong antimicrobial activity against multidrug-resistant bacteria and fungi and was not toxic to G. mellonella larvae up to a concentration of 500 mM. These results suggest that IbKTP-NH2, in addition to its known effect on cell membranes, can elicit a cellular immune response and, therefore, is promising for biomedical application.
Source: Fungal Biology - Category: Biology Source Type: research

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Authors: Chakrabarti A, Singh S Abstract Introduction: Since the emergence of Candida auris infection in 2009, the disease has been reported from multiple countries within a decade. The infection is classified under urgent threat, as it is multi-drug resistant, causes high mortality, spreads easily in hospital setting and is difficult to identify. Whole-genome sequencing has provided insights into this organisms biology and epidemiology. A comprehensive review of those issues would help the clinicians and scientists facing C. auris infection.Areas covered: We reviewed the epidemiological trends of C. auris infectio...
Source: Expert Review of Anti-Infective Therapy - Category: Infectious Diseases Tags: Expert Rev Anti Infect Ther Source Type: research
Candida auris is a multidrug-resistant yeast which has emerged in health care facilities worldwide; however, little is known about identification methods, patient colonization, environmental survival, spread, and drug resistance. Colonization on both biotic (patients) and abiotic (health care objects) surfaces, along with travel, appear to be the major factors for the spread of this pathogen across the globe. In this investigation, we present laboratory findings from an ongoing C. auris outbreak in New York (NY) from August 2016 through 2018. A total of 540 clinical isolates, 11,035 patient surveillance specimens, and 3,67...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Mycology Source Type: research
This article explores new perspectives on the mechanisms of antifungal acridine-peptide conjugates and acridine-based hybrid molecules to effectively combat fungal infections.
Source: Molecules - Category: Chemistry Authors: Tags: Review Source Type: research
In conclusion, new azole formulations with significantly enhanced activities against C. albicans and C. auris, while maintaining prolonged action and no toxicities at lower concentrations, were developed.
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Abstract The present work investigated the effect of camel's fetal fluids on a variety of bacterial and fungal pathogens. Ten samples of camel's amniotic and allantoic fluids were collected aseptically during parturition and their antimicrobial activities were evaluated by disc diffusion method and minimum inhibitory concentration (MIC) assay. The majority of tested pathogens were inhibited by both fluids up to 25% concentration. The fluids showed zones of inhibition ranging from 8 to 30 mm. The most pronounced inhibition was detected for Staphylococcus aureus, Listeria monocytogenes, Klebsiella pneumonia, an...
Source: Current Microbiology - Category: Microbiology Authors: Tags: Curr Microbiol Source Type: research
This article has been withdrawn by the authors. The +Glc image from the TMPM12 panel in Fig. 1E was reused in Fig. S2C as TMPM9+Glc. The AD-CDR1 image in Fig. 6B was reused in Fig. S2B as TMPM8+Glc. The AD-CDR1+TMPM8 image in Fig. 6B was reused in Fig. S2B as TMPM4+Glc. The AD-CDR1+TMPM8+Glc image in Fig. 6B was reused in Fig. S2C as TMPM5. The AD-CDR2 image in Fig. 6C was reused in Fig. S2B as TMPM8 and TMPM11+Glc. The AD-CDR2+TMPM8 image in Fig. 6C was reused in Fig. 8Bii as M1234A and N1240A and in Fig. S2B as TMPM1. A portion of the AD-CaMDR1 image in Fig. 6D was reused in Fig. S2B as TMPM2. The AD-CaMDR1+TMPM8 in Fig....
Source: Journal of Biological Chemistry - Category: Chemistry Authors: Tags: Withdrawals/Retractions Source Type: research
Conclusions: Gram-negative bacteria are the main causative agents of VAP cases, which mostly are MDR. Microorganisms likeC. pauculus andA. salmonicida should be taken into consideration as VAP causative agents. There was no common source of infection suggesting likely endogenous sources ofK. pneumoniae, the main causative agent of VAP in this study.
Source: The Journal of Infection in Developing Countries - Category: Infectious Diseases Source Type: research
Although multidrug-resistant Candida auris infections are rare, New York health officials advise close monitoring of cases after 3 patients in the state were found to have C auris that was resistant to all 3 classes of commonly prescribed antifungal drugs (pan resistance).
Source: JAMA - Category: General Medicine Source Type: research
Recent years have seen an unprecedented rise in the incidence of multidrug-resistant (MDR) Gram-negative bacteria (GNBs) such as Acinetobacter and Klebsiella species. In view of the shortage of novel drugs in the pipeline, alternative strategies to prevent, and treat infections by GNBs are urgently needed. Previously, we have reported that the Candida albicans hypha-regulated protein Hyr1 shares striking three-dimensional structural homology with cell surface proteins of Acinetobacter baumannii. Moreover, active vaccination with rHyr1p-N or passive immunization with anti-Hyr1p polyclonal antibody protects mice from Acineto...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
In this study, the 10 multidrug resistance transporters of the Drug:H+ Antiporter family of C. glabrata were screened for a role in biofilm formation. Besides previously identified players in this process, namely CgTpo1_2 and CgQdr2, two others are shown to contribute to biofilm formation: CgDtr1 and CgTpo4. The deletion of each of these genes was found to lead to lower biofilm formation, in both SDB and RPMI media, while their expression was found to increase during biofilm development and to be controlled by the transcription factor CgTec1, a predicted key regulator of biofilm formation. Additionally, the deletion of CgD...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
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