Light Sensing in Plant- and Rock-associated Black Fungi
Publication date: Available online 25 January 2020Source: Fungal BiologyAuthor(s): Julia Schumacher, Anna GorbushinaAbstractFungi that share light-flooded habitats with phototrophs may profit from their excess photosynthetic products. But to cope with sunlight-associated stresses [e.g. high temperatures, UV radiation with associated DNA damage, accumulation of reactive oxygen species (ROS), desiccation and osmotic stresses] it is important for fungi to accurately sense and respond to changes in light. To test the hypothesis that light is an environmental cue that Ascomycota use to coordinate growth, stress responses as wel...
Source: Fungal Biology - January 26, 2020 Category: Biology Source Type: research

Thoughts on the evolution of Core Environmental Responses in yeasts
Publication date: Available online 16 January 2020Source: Fungal BiologyAuthor(s): Alistair J.P. Brown, Daniel E. Larcombe, Arnab PradhanAbstractThe model yeasts, Saccharomyces cerevisiae and Schizosaccharomyces pombe, display Core Environmental Responses (CERs) that include the induction of a core set of stress genes in response to diverse environmental stresses. CERs underlie the phenomenon of stress cross-protection, whereby exposure to one type of stress can provide protection against subsequent exposure to a second type of stress. CERs have probably arisen through the accumulation, over evolutionary time, of protectiv...
Source: Fungal Biology - January 17, 2020 Category: Biology Source Type: research

Serendipity in the wrestle between Trichoderma and Metarhizium
Publication date: Available online 16 January 2020Source: Fungal BiologyAuthor(s): Edgar Q.A. Medina, Ariel S. Oliveira, Humberto R. Medina, Drauzio E.N. RangelAbstractThe fungal species Trichoderma is frequently found in soil antagonizing plant-pathogenic fungi as well as parasitizing plant-pathogenic nematodes. Metarhizium species are insect-pathogenic fungi that are used throughout the world to control agricultural insect pests. Here, we determine whether the antagonism (A) of T. atroviride to M. robertsii during growth and spore formation can impact the stress biology of M. robertsii conidia. Cultures of M. robertsii w...
Source: Fungal Biology - January 17, 2020 Category: Biology Source Type: research

Identification and characterization of a DevR-interacting protein in Aspergillus oryzae
In this study, to further explore the function of DevR, its interaction proteins were screened by a yeast two-hybrid assay. An A. oryzae cDNA library was transformed into the Y187 strain by using the SMART technique and the homologous recombination method, and then hybridized witha constructed DevR bait plasmid introducing strain to obtain positive clones. Through sequencing analysis, the potential interaction proteins of DevR were determined. Among them, an AO090701000363 gene-encoding protein (named DipA), which was predicted to be a basic leucine zipper (bZIP) transcription factor, was a possible candidate. Phenotypic a...
Source: Fungal Biology - January 15, 2020 Category: Biology Source Type: research

On the role of the global regulator RlcA in red-light sensing in Aspergillus nidulans
Publication date: Available online 10 January 2020Source: Fungal BiologyAuthor(s): Zhenzhong Yu, Jennifer Huebner, Satur Herrero, Victor Gourain, Reinhard FischerAbstractA large proportion of fungal genomes are under the control of light. Most fungi employ complex light sensing systems, consisting of red-, blue, and in some cases green-light photoreceptors. Here we studied the light response in Aspergillus nidulans. In a genetic screen, followed by whole-genome sequencing we identified a global regulator, which appears to be involved in chromatin structure modification. We therefore named the protein RlcA (regulator of lig...
Source: Fungal Biology - January 11, 2020 Category: Biology Source Type: research

Characterization of brown film formed by Lentinula edodes
This study provides a cytological foundation for evaluating the regulation of brown film formation in L. edodes. (Source: Fungal Biology)
Source: Fungal Biology - January 9, 2020 Category: Biology Source Type: research

Genome comparison and transcriptome analysis of the invasive brown root rot pathogen, Phellinus noxius, from different geographic regions reveals potential enzymes associated with degradation of different wood substrates
This study highlights the vast suite of decomposing enzymes produced by P. noxius, which suggests potential for degrading diverse wood substrates, even from temperate host trees. This information contributes to our understanding of pathogen ecology, mechanisms of wood decomposition, and pathogenic/saprophytic lifestyle. (Source: Fungal Biology)
Source: Fungal Biology - January 9, 2020 Category: Biology Source Type: research

Transcriptomic analysis reveals global and temporal transcription changes during Candida glabrata adaptation to an oxidative environment
Publication date: Available online 28 December 2019Source: Fungal BiologyAuthor(s): Pooja Sethiya, Maruti Nandan Rai, Rikky Rai, Chirag Parsania, Kaeling Tan, Koon Ho WongAbstractThe ability to survive host-elicited oxidative stress is critical for microbial pathogens to cause infection. The human fungal pathogen Candida glabrata can tolerate high levels of oxidative stress and proliferate inside phagocytes. Previous studies had successfully identified a transcription response to oxidative stress including induction of a core set of detoxification genes. However, the findings only represent an early snapshot of a highly dy...
Source: Fungal Biology - December 29, 2019 Category: Biology Source Type: research

Editorial Board
Publication date: January 2020Source: Fungal Biology, Volume 124, Issue 1Author(s): (Source: Fungal Biology)
Source: Fungal Biology - December 29, 2019 Category: Biology Source Type: research

Colonization of Metarhizium anisopliae on the surface of pine tree logs: a promising biocontrol strategy for the Japanese pine sawyer, Monochamus alternatus
Publication date: Available online 26 December 2019Source: Fungal BiologyAuthor(s): Jong Cheol Kim, Sehyeon Baek, So Eun Park, Sihyeon Kim, Mi Rong Lee, Minsung Jo, Jae Seong Im, Panjung Ha, Jae Su Kim, Tae Young ShinAbstractWe investigated the colonization potential of five Metarhizium anisopliae isolates on pine tree surfaces under laboratory conditions, determined the influence of the pine bark extract on fungal growth and evaluated the insecticidal activity following colonization on the Japanese pine sawyer. Finally, the effect of colonization on adults pine sawyer was evaluated using the top three performing isolates ...
Source: Fungal Biology - December 27, 2019 Category: Biology Source Type: research

Phylogenetic re-evaluation of the Grosmannia penicillata complex (Ascomycota, Ophiostomatales), with the description of five new species from China and USA
Publication date: Available online 25 December 2019Source: Fungal BiologyAuthor(s): Mingliang Yin, Michael J. Wingfield, Xudong Zhou, Z. Wilhelm de BeerAbstractThe Grosmannia penicillata complex (Ophiostomatales, Ascomycota) is one of the major species complexes in Leptographium sensu lato. Most of these are wood staining fungi associated with conifer-infesting bark beetles, and the complex encompasses the type species of the genus Grosmannia. Yet the phylogenetic relationships of species within the complex is unresolved. The aim of this study was to re-evaluate the circumscriptions of all known species in the G. penicilla...
Source: Fungal Biology - December 26, 2019 Category: Biology Source Type: research

Multiple virus infections on Heterobasidion sp.
Publication date: Available online 23 December 2019Source: Fungal BiologyAuthor(s): Jarkko Hantula., Salla Mäkelä, Ping Xu, Veera Brusila, Heikki Nuorteva, Muhammad Kashif, Rafiqul Hyder, Eeva J. VainioAbstractHeterobasidion viruses have previously been shown to affect each other’s transmission and phenotypic effects on their hosts in a complex way. In this work, Heterobasidion parviporum strains hosting five coinfecting viruses simultaneously were constructed and used as donors in transmission experiments. They showed that viruses move more frequently between the mycelia of the same species than between th...
Source: Fungal Biology - December 24, 2019 Category: Biology Source Type: research

Antifungal and anti-biofilm activity of designed derivatives from Kyotorphin
This study assessed the antimicrobial and antibiofilm activity of these peptides. The antifungal activity of kyotorphins was determined in representative strains of Candida species, including C. albicans ATCC 10231, C. krusei ATCC 6258, and six clinical isolates—C. dubliniensis 19-S, C. glabrata 217-S, C. lusitaniae 14-S, C. novergensis 51-S, C. parapsilosis 63, and C. tropicalis 140-S—obtained from the oral cavity of HIV-positive patients. The peptides were synthesized by standard solution or solid-phase synthesis, purified by RP-HPLC (purity>95%), and characterized by nuclear magnetic resonance. The result...
Source: Fungal Biology - December 20, 2019 Category: Biology Source Type: research

The extraradical proteins of Rhizophagus irregularis: a shotgun proteomics approach
Publication date: Available online 10 December 2019Source: Fungal BiologyAuthor(s): Chelsea L. Murphy, Noha H. Youssef, Steve Hartson, Mostafa S. ElshahedAbstractArbuscular Mycorrhizal fungi (AMF, Glomeromycota) form obligate symbiotic associations with the roots of most terrestrial plants. Our understanding of the molecular mechanisms enabling AMF propagation and AMF-host interaction is currently incomplete. Analysis of AMF proteomes could yield important insights and generate hypotheses on the nature and mechanism of AMF-plant symbiosis. Here, we examined the extraradical mycelium proteomic profile of the arbuscular myco...
Source: Fungal Biology - December 11, 2019 Category: Biology Source Type: research

Differences in gene modulation in Saccharomyces cerevisiae indicate that maturity plays an important role in the high hydrostatic pressure stress response and resistance
Publication date: Available online 11 December 2019Source: Fungal BiologyAuthor(s): Brígida A.A. Spagnol, Tathiana F.S. Antunes, Oeber F. Quadros, A. Alberto R. Fernandes, Patricia M.B. FernandesAbstractThere is a strong relationship between the regulatory pathways to oxidative stress, longevity, and aging. High hydrostatic pressure (HHP) induces oxidative stress and activates cellular defense mechanisms. The understanding of these mechanisms is a strategy to delay damage associated with aging. Addressing resistance to stress and aging in Saccharomyces cerevisiae is a well-accepted approach since pathways involved i...
Source: Fungal Biology - December 11, 2019 Category: Biology Source Type: research

Potential use of machine learning methods in assessment of Fusarium culmorum and Fusarium proliferatum growth and mycotoxin production in treatments with antifungal agents
Publication date: Available online 22 November 2019Source: Fungal BiologyAuthor(s): Andrea Tarazona, Eva M. Mateo, José V. Gómez, David Romera, Fernando MateoAbstractFusarium-controlling fungicides are necessary to limit crop loss. Little is known about the effect of antifungal formulations at sub-lethal doses, and their interaction with abiotic factors, on Fusarium culmorum and F. proliferatum development and on zearalenone and fumonisin biosynthesis, respectively. In the present study different treatments based on sulfur, trifloxystrobin and demethylation inhibitor fungicides (cyproconazole, tebuconazo...
Source: Fungal Biology - December 11, 2019 Category: Biology Source Type: research

Hydrosoluble phylloplane components of Theobroma cacao modulate the metabolism of Moniliophthora perniciosa spores during germination
Publication date: Available online 5 December 2019Source: Fungal BiologyAuthor(s): Joise Hander Mares, Karina Peres Gramacho, Juliano Oliveira Santana, Aurizângela Oliveira de Souza, Fátima Cerqueira Alvim, Carlos Priminho PirovaniAbstractThe surface of plants forms a defense barrier that directly inhibits the first point of contact of microorganisms with the host. To understand this defense mechanism in Moniliophthora perniciosa interaction with Theobroma cacao cv Catongo, the aim of this study was to compare the changes in protein expression in basidiospores of the fungus M. perniciosa in response the leaf w...
Source: Fungal Biology - December 5, 2019 Category: Biology Source Type: research

Quercetin-induced yeast apoptosis through mitochondrial dysfunction under the accumulation of magnesium in Candida albicans
Publication date: Available online 2 December 2019Source: Fungal BiologyAuthor(s): Min Seok Kwun, Dong Gun LeeAbstractLatterly, the upsurge in use of antifungal drugs has brought about the emergence of several drug-resistance strains, making it skeptical to continue relying on current therapeutic regime. In the necessity of resistance-free antifungal agent, flavonoids presented possibilities of replacing existing drugs, displaying antifungal activity against pathogenic fungi. Among them, quercetin, one of the most representative flavonoids, exhibited antifungal activity against Candida albicans. To inspect the further unde...
Source: Fungal Biology - December 2, 2019 Category: Biology Source Type: research

Transcriptomes of the interaction between Fusarium verticillioides and a Streptomyces strain reveal the fungal defense strategy under the pressure of a potential biocontrol agent
Publication date: Available online 27 November 2019Source: Fungal BiologyAuthor(s): C. Strub, C.A.T. Dieye, P.A. Nguyen, F. Constancias, N. Durand, S. Guendouz, M. Pratlong, A. Fontana, S. Schorr-GalindoAbstractThe actinobacteria Streptomyces sp. AV05 isolated from an organic amendment appears to be a potential biocontrol agent (BCA) against mycotoxigenic fungi. It was found to significantly inhibit Fusarium verticillioides growth and mycotoxin production during their co-cultivation on Petri dishes. F. verticillioides growth was durably affected while the decrease of the toxin production levels was reversible, suggesting d...
Source: Fungal Biology - November 28, 2019 Category: Biology Source Type: research

Potential use of machine learning methods in assessment of Fusarium culmorum and F. proliferatum growth and mycotoxin production in treatments with antifungal agents
Publication date: Available online 22 November 2019Source: Fungal BiologyAuthor(s): Andrea Tarazona, Eva M. Mateo, José V. Gómez, David Romera, Fernando MateoAbstractThe use of Fusarium-controlling fungicides is necessary to limit crop loss. Little is known about the effect of commercial antifungal formulations at sub-lethal doses, and their interaction with abiotic factors, on Fusarium culmorum and F. proliferatum development and on zearalenone and fumonisin biosynthesis, respectively. In the present study different treatments based on sulfur, trifloxystrobin and demethylation inhibitor fungicides (cyprocona...
Source: Fungal Biology - November 22, 2019 Category: Biology Source Type: research

Radioadapted Wangiella dermatitidis senses radiation in its environment in a melanin-dependent fashion
Publication date: Available online 12 November 2019Source: Fungal BiologyAuthor(s): Mackenzie E. Malo, Connor Frank, Ekaterina DadachovaAbstractBlack fungi withstand extreme stresses partly due to the presence of melanin. Melanin is associated with structural integrity and resistance to chemical and radiation stress. This results in improved health and fitness, specifically in extreme conditions. Our goal was to exploit the radiation sensing nature of melanized fungus in order to develop a radioadapted strain capable of responding to radiation in the environment. The protracted exposure of a melanized fungus, Wangiell...
Source: Fungal Biology - November 21, 2019 Category: Biology Source Type: research

Extracellular peptidases of insect-associated fungi and their possible use in biological control programs and as pathogenicity markers
Publication date: Available online 20 November 2019Source: Fungal BiologyAuthor(s): Tatyana A. Semenova, Yakov E. Dunaevsky, Galina A. Beljakova, Mikhail A. BelozerskyAbstractThis review deals with characteristics of peptidases of fungi whose life cycles are associated with insects to varying degrees. The review examines the characteristic features of the extracellular peptidases of entomopathogenic fungi, the dependence of the specificity of these peptidases on the ecological characteristics of the fungi, and the role of peptidases in the development of the pathogenesis. Data on the properties and physiological role of hy...
Source: Fungal Biology - November 21, 2019 Category: Biology Source Type: research

Evaluating the circadian rhythm and response to glucose addition in dispersed growth cultures of Neurospora crassa
Publication date: Available online 20 November 2019Source: Fungal BiologyAuthor(s): Christina M. Kelliher, Jennifer J. Loros, Jay C. DunlapAbstractWork on the filamentous fungus Neurospora crassa has contributed to or pioneered many aspects of research on circadian clock mechanism, a process that is functionally conserved across eukaryotes. Biochemical assays of the fungal circadian clock typically involve growth in liquid medium where Neurospora forms a spherical ball of submerged mycelium. Here, we revive a method for dispersed growth of Neurospora in batch culture using polyacrylic acid as an additive to the medium. We ...
Source: Fungal Biology - November 21, 2019 Category: Biology Source Type: research

Identification of two Pleurotus ostreatus blue light receptor genes (PoWC-1 and PoWC-2) and in vivo confirmation of complex PoWC-12 formation through yeast two hybrid system
In this study, we isolated homologues of the blue light receptor genes wc-1 and wc-2 from P. ostreatus, PoWC-1 and PoWC-2. The PoWC-1 contained three typical PAS domains and one PAS domain exhibited significant similarity to the LOV domain of known blue light receptors. The PoWC-2 had one typical PAS domain and one ZnF domain. The qRT-PCR analysis showed that PoWC-1 and PoWC-2 expression increased in a short time, and the final level tended to be stable along with the light illumination. The PoWC-1 and PoWC-2 expression levels of the primordium period was higher than that of mature fruiting-body period; and in the pil...
Source: Fungal Biology - November 16, 2019 Category: Biology Source Type: research

A combined transcriptional, biochemical and histopathological study unravels the complexity of Alternaria resistance and susceptibility in Brassica coenospecies
In this study, we have selected B. juncea (susceptible), Sinapis alba (moderately tolerant) and Camelina sativa (highly resistant) as a source for understanding and unraveling the mechanism of disease resistance against A. brassicae. Histopathological studies showed early onset of necrosis in B. juncea (1day post inoculation) and delayed in S. alba (2 dpi) and C. sativa (3 dpi) respectively. Based on the biochemical observations, early and enhanced production of hydrogen peroxide (H2O2) was observed in C. sativa and S. alba (6 hpi) when compared to B. juncea (12 hpi) after A. brassicae infection. An increase in catalase ac...
Source: Fungal Biology - November 15, 2019 Category: Biology Source Type: research

Botrydial confers Botrytis cinerea the ability to antagonize soil and phyllospheric bacteria
Publication date: Available online 15 November 2019Source: Fungal BiologyAuthor(s): Paulina Vignatti, María E. Gonzalez, Edgardo C. Jofré, Hernando J. Bolívar-Anillo, Javier Moraga, Muriel Viaud, Isidro G. Collado, Fernando L. PieckenstainAbstractThe role of the sesquiterpene botrydial in the interaction of the phytopathogenic fungus Botrytis cinerea and plant-associated bacteria was analyzed. From a collection of soil and phyllospheric bacteria, nine strains sensitive to growth-inhibition by B. cinerea were identified. B. cinerea mutants unable to produce botrydial caused no bacterial inhibition, thus...
Source: Fungal Biology - November 15, 2019 Category: Biology Source Type: research

Editorial Board
Publication date: December 2019Source: Fungal Biology, Volume 123, Issue 12Author(s): (Source: Fungal Biology)
Source: Fungal Biology - November 15, 2019 Category: Biology Source Type: research

Radioadapted Wangiella dermatitdis Senses Radiation in its Environment in a Melanin-dependent Fashion
Publication date: Available online 12 November 2019Source: Fungal BiologyAuthor(s): Mackenzie E. Malo, Connor Frank, Ekaterina DadachovaAbstractBlack fungi have the ability to withstand varied and extreme stresses, in part due to the presence of the pigment melanin. Melanin contributes to increased structural integrity and enhanced resistance to chemical and radiation stress. These advantages impart biological benefit in the form of improved health and fitness, specifically in extreme conditions. Our goal in this study was to exploit the radiation sensing nature of melanized fungus in order to develop a radioadapted strain...
Source: Fungal Biology - November 13, 2019 Category: Biology Source Type: research

Interacting climate change factors (CO2 and temperature cycles) effects on growth, secondary metabolite gene expression and phenotypic ochratoxin A production by Aspergillus carbonarius strains on a grape-based matrix
This study examined the effect of (i) current and increased temperature in the alternating 11.5h dark/12.5h light cycle (15-28°C vs 18-34°C), representative of the North Apulia area, South Italy and (ii) existing and predicted CO2 concentrations (400 vs 1000 ppm), on growth, expression of biosynthetic genes (AcOTApks, AcOTAnrps, AcOTAhal, AcOTAp450, AcOTAbZIP) and regulatory genes of Velvet complex (laeA/veA/velB, “velvet complex”) involved in OTA biosynthesis and OTA phenotypic production by three strains of A. carbonarius. The experiments made on a grape-based matrix showed that elevated CO2 resulted ...
Source: Fungal Biology - November 13, 2019 Category: Biology Source Type: research

Four Tulasnella taxa associated with populations of the Australian evergreen terrestrial orchid Cryptostylis ovata
Publication date: Available online 23 October 2019Source: Fungal BiologyAuthor(s): D.Q. Nguyen, Hua Li, T.T. Tran, K. Sivasithamparam, M.G.K. Jones, S.J. WylieAbstractOf the more than 400 indigenous orchid species in Western Australia, Cryptostylis ovata is the only species that retains its leaves all year round. It exists as a terrestrial herb and occasionally as an epiphyte in forested areas. Like all terrestrial orchids, C. ovata plants associate with mycorrhizal fungi, but their identities have not previously been investigated. Fungi were isolated from pelotons in rhizomes collected from three southern and tw...
Source: Fungal Biology - November 9, 2019 Category: Biology Source Type: research

Mitigating stress in industrial yeasts
This article will highlight the importance of furthering our understanding of key aspects of yeast stress physiology and the beneficial impact this can have more generally on enhancing industrial fungal bioprocesses. (Source: Fungal Biology)
Source: Fungal Biology - November 5, 2019 Category: Biology Source Type: research

Identification of two Pleurotus ostreatus blue light receptor genes (PoWC-1 and PoWC-2) and in vivo confirmation of complex PoWC-12 formation through yeast two hybrid system
In this study, we isolated homologues of the blue light receptor genes wc-1 and wc-2 from P. ostreatus, PoWC-1 and PoWC-2. The PoWC-1 contained three typical PAS domains and one PAS domain exhibited significant similarity to the LOV domain of known blue light receptors. The PoWC-2 had one typical PAS domain and one ZnF domain. The qRT-PCR analysis showed that PoWC-1 and PoWC-2 expression increased in a short time, and the final level tended to be stable along with the light illumination. The PoWC-1 and PoWC-2 expression levels of the primordium period was higher than that of mature fruiting-body period; and in the pileus w...
Source: Fungal Biology - November 3, 2019 Category: Biology Source Type: research

Bacterial decomposition of insects post-Metarhizium infection: Possible influence on plant growth
Publication date: Available online 15 October 2019Source: Fungal BiologyAuthor(s): Vadim Y. Kryukov, Marsel R. Kabilov, Natalya Smirnova, Oksana G. Tomilova, Maksim V. Tyurin, Yuriy B. Akhanaev, Olga V. Polenogova, Viktor P. Danilov, Saule K. Zhangissina, Tatiana Alikina, Olga N. Yaroslavtseva, Viktor V. GlupovAbstractStrains of entomopathogenic fungi may have substantial differences in their final stages of mycosis. Insect cadavers are usually overgrown with mycelium after colonization of the insect body, but in many cases, bacterial decomposition of the colonized hosts occurs. We used two Metarhizium robertsii strains in...
Source: Fungal Biology - October 31, 2019 Category: Biology Source Type: research

Crosstalk between Saccharomyces cerevisiae SAPKs Hog1 and Mpk1 is mediated by glycerol accumulation
In this study, we show that hyperactivation of Mpk1 in a ptp2 ptp3 null mutant is an indirect consequence of Hog1 hyperactivation, which induces accumulation of intracellular glycerol and an attendant hypo-osmotic stress. Mpk1 hyperactivity in the absence of PTP2 and PTP3 was suppressed by a hog1 null mutation, or by restoration of osmotic balance with a constitutive form of the glycerol channel Fps1. We found similarly that activation of Mpk1 in response to zymolyase treatment is partly a consequence of Hog1-driven glycerol accumulation. Thus, we have identified two conditions in which glycerol serves as a mediator of cro...
Source: Fungal Biology - October 29, 2019 Category: Biology Source Type: research

General stress response or adaptation to rapid growth in Aspergillus nidulans?
Publication date: Available online 28 October 2019Source: Fungal BiologyAuthor(s): Károly Antal, Barnabás Cs. Gila, István Pócsi, Tamás EmriAbstractGenome-wide transcriptional changes in Aspergillus nidulans induced by nine different stress conditions were evaluated to reveal the general environmental stress response gene set showing unidirectional expressional changes under various types of stress. Clustering the genes by their transcriptional changes was a useful technique for identifying large groups of co-regulated genes. Altogether, 1642 co-upregulated and 3916 co-downregulated genes...
Source: Fungal Biology - October 29, 2019 Category: Biology Source Type: research

Gene expression varies within and between enzootic and epizootic lineages of Batrachochytrium dendrobatidis (Bd) in the Americas
Publication date: Available online 24 October 2019Source: Fungal BiologyAuthor(s): C.A. McDonald, A.R. Ellison, L.F. Toledo, T.Y. James, K.R. ZamudioAbstractWhile much research focus is paid to hypervirulent fungal lineages during emerging infectious disease outbreaks, examining enzootic pathogen isolates can be equally fruitful in delineating infection dynamics and determining pathogenesis. The fungal pathogen of amphibians, Batrachochytrium dendrobatidis (Bd), exhibits markedly different patterns of disease in natural populations, where it has caused massive amphibian declines in some regions, yet persists enzootically i...
Source: Fungal Biology - October 26, 2019 Category: Biology Source Type: research

Carbon dioxide production as an indicator of Aspergillus flavus colonisation and aflatoxins/cyclopiazonic acid contamination in shelled peanuts stored under different interacting abiotic factors
This study evaluated the relationship between storage of shelled peanuts under interacting abiotic conditions on (a) the temporal colonisation kinetics and cumulative respiration rates, (b) quantified the effect on dry matter losses (DMLs) and (c) quantify accumulation of aflatoxin B1 (AFB1) and CPA. Both naturally contaminated and inoculated peanuts with A. flavus conidia were stored for 7-days under different water activity (aw; 0.77-0.95) and temperature levels (20-35°C). Gas Chromatography (GC-TCD) was used to measure the temporal respiration rates (R) and the total accumulated CO2 production. Mycotoxin contaminati...
Source: Fungal Biology - October 26, 2019 Category: Biology Source Type: research

Four Tulasnella species are associated with populations of the Australian evergreen terrestrial orchid Cryptostylis ovata
Publication date: Available online 23 October 2019Source: Fungal BiologyAuthor(s): D.Q. Nguyen, Hua Li, T.T. Tran, K. Sivasithamparam, M.G.K. Jones, S.J. WylieAbstractOf the more than 400 indigenous orchid species in Western Australia, Cryptostylis ovata is the only species that retains its leaves all year round. It exists as a terrestrial herb and occasionally as an epiphyte in forested areas. Like all terrestrial orchids, C. ovata plants associate with mycorrhizal fungi, but their identities have not previously been investigated. Fungi were isolated from pelotons in rhizomes collected from three southern and two northern...
Source: Fungal Biology - October 23, 2019 Category: Biology Source Type: research

Light regulates a Phycomyces blakesleeanus gene family similar to the carotenogenic repressor gene of Mucor circinelloides
Publication date: Available online 23 October 2019Source: Fungal BiologyAuthor(s): Víctor G. Tagua, Eusebio Navarro, Gabriel Gutiérrez, Victoriano Garre, Luis M. CorrochanoAbstractThe transcription of about 5-10% of the genes in Phycomyces blakesleeanus is regulated by light. Among the most up-regulated we have identified four genes, crgA-D, with similarity to crgA of Mucor circinelloides. The crgA gene encodes a repressor of light-inducible carotenogenesis. The four proteins have the same structure with two Ring Finger domains and a LON domain, suggesting that they could act as ubiquitin ligases, as their M....
Source: Fungal Biology - October 23, 2019 Category: Biology Source Type: research

Insight into a highly polymorphic endophyte isolated from the roots of the halophytic seepweed Suaeda salsa: Laburnicola rhizohalophila sp. nov. (Didymosphaeriaceae, Pleosporales)
Publication date: Available online 16 October 2019Source: Fungal BiologyAuthor(s): Zhilin Yuan, Irina S. Druzhinina, Xinyu Wang, Xiaoguo Zhang, Long Peng, Jessy LabbéAbstractWe surveyed root endophytic fungi of the coastal halophyte Suaeda salsa and detected a population of a novel species that we described here as Laburnicola rhizohalophila sp. nov. No sexual sporulating structure was observed. Instead, it produced a large amount of thalloconidia, 0-1 transverse septa, hyaline to darkly pigmented, often peanut-shaped and sometimes dumbbell-shaped, both ends enlarged with numerous oil droplets inside the hyphal cell...
Source: Fungal Biology - October 19, 2019 Category: Biology Source Type: research

Metabolic coupling in the co-cultured fungal-yeast suite of Trametes ljubarskyi and Rhodotorula mucilaginosa leads to hypersecretion of laccase isozymes
This study allows us to deepen and refine our understanding of fungal-yeast symbiotic interaction; further, it also establishes a mutual relation by metabolic coupling for 10-fold higher laccase isozyme secretion (6532 U/ml). The purified laccase isozymes showed acidic pH optima (pH 3–4), higher thermo-stability (60 °C), and broad enzyme kinetics (Km) values. Our study also provides an in-depth understanding of laccase isozymes and their potential to degrade synthetic dyes, which may help the fungi to survive in an adverse environment. (Source: Fungal Biology)
Source: Fungal Biology - October 19, 2019 Category: Biology Source Type: research

In Saccharomyces cerevisiae, withdrawal of the carbon source results in detachment of glycolytic enzymes from the cytoskeleton and in actin reorganization
Publication date: Available online 17 October 2019Source: Fungal BiologyAuthor(s): E. Espinoza-Simón, N. Chiquete-Félix, L. Morales-García, U. Pedroza-Dávila, X. Pérez-Martínez, D. Araiza-Olivera, F. Torres-Quiroz, S. Uribe-CarvajalAbstractMetabolons are dynamic associations of enzymes catalyzing consecutive reactions within a given pathway. Association results in enzyme stabilization and increased metabolic efficiency. Metabolons may use cytoskeletal elements, membranes and membrane proteins as scaffolds. The effects of glucose withdrawal on a putative glycolytic metabolon/F-actin...
Source: Fungal Biology - October 19, 2019 Category: Biology Source Type: research

Bacterial decomposition of insects post-Metarhizium infection, possible influence on plant growth
Publication date: Available online 15 October 2019Source: Fungal BiologyAuthor(s): Vadim Y. Kryukov, Marsel R. Kabilov, Natalia Smirnova, Oksana G. Tomilova, Maksim V. Tyurin, Yuriy B. Akhanaev, Olga V. Polenogova, Viktor P. Danilov, Saule K. Zhangissina, Tatiana Alikina, Olga N. Yaroslavtseva, Viktor V. GlupovAbstractStrains of entomopathogenic fungi may have substantial differences in their final stages of mycosis. Insect cadavers are usually overgrown with mycelium after colonization of the insect body, but in many cases, bacterial decomposition of the colonized hosts occurs. We used two Metarhizium robertsii strains in...
Source: Fungal Biology - October 16, 2019 Category: Biology Source Type: research

Crosstalk between S. cerevisiae SAPKs Hog1 and Mpk1 is mediated by glycerol accumulation
In this study, we show that hyperactivation of Mpk1 in a ptp2 ptp3 null mutant is an indirect consequence of Hog1 hyperactivation, which induces accumulation of intracellular glycerol and an attendant hypo-osmotic stress. Mpk1 hyperactivity in the absence of PTP2 and PTP3 was suppressed by a hog1 null mutation, or by restoration of osmotic balance with a constitutive form of the glycerol channel Fps1. We found similarly that activation of Mpk1 in response to zymolyase treatment is partly a consequence of Hog1-driven glycerol accumulation. Thus, we have identified two conditions in which glycerol serves as a mediator of cro...
Source: Fungal Biology - October 16, 2019 Category: Biology Source Type: research

Editorial Board
Publication date: November 2019Source: Fungal Biology, Volume 123, Issue 11Author(s): (Source: Fungal Biology)
Source: Fungal Biology - October 16, 2019 Category: Biology Source Type: research

Characterization of gfdb, putatively encoding a glycerol 3-phosphate dehydrogenase in Aspergillus nidulans
Publication date: Available online 11 October 2019Source: Fungal BiologyAuthor(s): Anita Király, Csaba Hámori, Gyöngyi Gyémánt, Katalin E. Kövér, István Pócsi, Éva LeiterAbstractThe genome of Aspergillus nidulans accommodates two glycerol 3-phosphate dehydrogenase genes, gfdA and gfdB. Previous studies confirmed that GfdA is involved in the osmotic stress defence of the fungus. In this work, the physiological role of GfdB was characterized via the construction and functional characterization of the gene deletion mutant ΔgfdB. Unexpectedly, ΔgfdB ...
Source: Fungal Biology - October 13, 2019 Category: Biology Source Type: research

High Specificity Of A Rare Terrestrial Orchid Toward A Rare Fungus Within The North American Tallgrass Prairie
Publication date: Available online 4 October 2019Source: Fungal BiologyAuthor(s): Jaspreet Kaur, Lela Andrews, Jyotsna SharmaAbstractThe Orchidaceae are globally distributed and represent a diverse lineage of obligate mycotrophic plants. Given their dependence upon specific fungi for seed germination or plant development, composition of soil fungal communities is expected to influence the distribution and persistence of orchid species though simultaneous characterization of orchid mycorrhizal fungal (OMF) communities in roots and in soil is rarely reported. To explain the OMF co-distributions in orchid roots, orchid occupi...
Source: Fungal Biology - October 5, 2019 Category: Biology Source Type: research

Fungal community associated with adults of the chestnut gall wasp Dryocosmus kuriphilus after emergence from galls: taxonomy and functional ecology
Publication date: Available online 4 October 2019Source: Fungal BiologyAuthor(s): Carmen Morales-Rodriguez, Ivano Sferrazza, MariaPia Aleandri, Matteo Dalla Valle, Tommaso Mazzetto, Stefano Speranza, Mario Contarini, Andrea VanniniAbstractThe diversity of the fungal community associated with adults of Dryocosmus kuriphilus following emergence was examined using HTS analysis. Ascomycota dominated the fungal core-biome community. The functional guilds of the 90 taxa forming the core-biome were assessed, demonstrating three main groups: saprotrophs, plant pathogens and entomopathogens. Twenty-nine OTUs out of 90 were resolved...
Source: Fungal Biology - October 5, 2019 Category: Biology Source Type: research

Adaptation of subpopulations of the Norway spruce needle endophyte Lophodermium piceae to the temperature regime
Publication date: Available online 28 September 2019Source: Fungal BiologyAuthor(s): Michael M. Müller, Leena Hamberg, Tatjana Morozova, Alexander Sizykh, Thomas SieberSummaryLophodermium piceae represents the most common Norway spruce needle endophyte. The aim of this study was to find out whether subpopulations of L. piceae in climatically different environments (in which Norway spruce occurs natively) are adapted to local thermal conditions. L. piceae’s ability for thermal adaptation was investigated by determining growth rates of 163 isolates in vitro at four different temperatures: 2, 6, 20 and 25°C. Is...
Source: Fungal Biology - September 29, 2019 Category: Biology Source Type: research

Antimicrobial and antioxidative potential of free and immobilised cellobiose dehydrogenase isolated from wood degrading fungi
Publication date: Available online 24 September 2019Source: Fungal BiologyAuthor(s): Justyna Sulej, Monika Osińska-Jaroszuk, Magdalena Jaszek, Marcin Grąz, Jolanta Kutkowska, Anna Pawlik, Agata Chudzik, Renata BancerzAbstractCellobiose dehydrogenase (CDH, EC 1.1.99.18) is a glycoprotein having many biotechnological applications. In the present study, CDHs isolated from Phlebia lindtneri (PlCDH), Phanerochaete chrysosporium (PchCDH), Cerrena unicolor (CuCDH), and Pycnoporus sanguineus (PsCDH) were studied the first time for their ability to generate antioxidant and antimicrobial agents. The aim of the research was to eval...
Source: Fungal Biology - September 26, 2019 Category: Biology Source Type: research