Propofol facilitates migration and invasion of oral squamous cell carcinoma cells by upregulating SNAI1 expression

Publication date: Available online 4 December 2019Source: Life SciencesAuthor(s): Chunzhu Li, Ming Xia, Hao Wang, Wenlong Li, Jiali Peng, Hong JiangAbstractAimTo investigate the effect of propofol on the migration and invasion of oral squamous cell carcinoma (OSCC) cells and explore the underlying mechanism.Main methodsCal-27 and SCC-25 cells treated with or without propofol, then the cells metastasis were determined. The levels of SNAI1 mRNA and protein were detected by real-time PCR and western blot. Cell migration ability was evaluated by wound healing assay, and the invasion of cells was measured using transwell assay.Key findingsPropofol treatment significantly promoted cell migration and invasion of OSCC. Further mechanistic studies of the stimulating effects of propofol on OSCC cell metastasis revealed that propofol treatment dose-dependently upregulated the expression of SNAI1, a member of the Snail superfamily of zinc-finger transcription factors. Additionally, the inhibition of endogenous SNAI1 expression reversed the effect of propofol on cell migration and invasion in Cal-27 and SCC-25 cells.SignificanceOur results demonstrate that propofol at clinically relevant concentrations facilitates cell migration and invasion through up-regulation of SNAI1 in OSCC cells, and suggest propofol may not be suitable for anesthesia management in OSCC patients.
Source: Life Sciences - Category: Biology Source Type: research

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GAS5, a FoxO1-actived long noncoding RNA, promotes propofol-induced oral squamous cell carcinoma apoptosis by regulating the miR-1297-GSK3β axis. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3985-3993 Authors: Gao C, Ren C, Liu Z, Zhang L, Tang R, Li X Abstract Propofol, an intravenous anaesthetic agent, has been found to exhibit antitumour effects in various kinds of cancer cells. However, the potential roles and regulatory mechanisms of propofol in oral squamous cell carcinoma (OSCC) remain unknown. Herein, we found that propofol inhibits OSCC cell growth and promotes cell apoptosis in a dose-...
Source: Artificial Cells, Nanomedicine and Biotechnology - Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research
Conclusion: Our data confirm the feasibility of free flaps for OSCC reconstruction in appropriately selected elderly patients. Preoperative assessment and aggressive management of glycemia in patients with diabetes is mandatory. DGA should be reduced as much as possible to prevent post-surgical complications. Comprehensive geriatric assessment is of paramount importance in this subset of patients.
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
We report an exceedingly rare case of SCC of the tongue arising in the midline of the dorsum in a 33-year-old man and review the literature. The patient was referred by a primary care physician and visited our hospital with a chief complaint of a mass in the midline of the tongue dorsum. Intraoral examination revealed a mass with a diameter of 14 × 13 mm anterior to the vallate papillae in the midline of the dorsum of the tongue. The mass was ellipse, well-circumscribed and elastic hard with no surrounding induration. There was no palpable enlarged lymph in the submandibular and cervical region. ...
Source: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology - Category: ENT & OMF Source Type: research
Authors: Okazaki S, Shintani S, Hirata Y, Suina K, Semba T, Yamasaki J, Umene K, Ishikawa M, Saya H, Nagano O Abstract The cystine-glutamate antiporter subunit xCT suppresses iron-dependent oxidative cell death (ferroptosis) and is therefore a promising target for cancer treatment. Given that cancer cells often show resistance to xCT inhibition resulting in glutathione (GSH) deficiency, however, we here performed a synthetic lethal screen of a drug library to identify agents that sensitize the GSH deficiency-resistant cancer cells to the xCT inhibitor sulfasalazine. This screen identified the oral anesthetic dyclon...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
Acantholytic squamous cell carcinoma (ASCC) is an uncommon aggressive histological variant of squamous cell carcinoma. The present report described the case of a 71-year-old man, referred to our service for examination of a painful swelling in the palate. An intraoral examination revealed an exophytic, sessile, red and white lesion with ulcerated areas on the hard and soft palate, extending to the left maxillary alveolar ridge and oropharynx. An incisional biopsy was performed under local anesthesia.
Source: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics - Category: ENT & OMF Authors: Tags: Oral and Maxillofacial Pathology Source Type: research
Publication date: Available online 5 April 2018 Source:British Journal of Oral and Maxillofacial Surgery Author(s): A.K. Bartella, A.-K. Sander, M. Kamal, J. Steegmann, A. Kloss-Brandstätter, J. Teichmann, F. Hölzle, B. Lethaus Despite improvements in the management of patients in critical care, about 3% patients who have an operation with curative intent for oral squamous cell carcinoma (SCC) do not survive their stay in hospital. Our aim was to assess the risk factors for postoperative death that were independent of the stage of the cancer, or the age and sex of the patients. We screened 4760 consecutive inpat...
Source: British Journal of Oral and Maxillofacial Surgery - Category: ENT & OMF Source Type: research
The objective of the present study was to investigate the effects of sevoflurane on the proliferation, apoptosis and invasion in HNSCC cell lines and the underlying molecular mechanism. The Cell Counting Kit-8 assay was used to evaluate cell proliferation. Apoptosis was analyzed by flow cytometry. Cell invasion was evaluated using the Transwell invasion assay. The expression levels of Akt, p-Akt (Ser473), hypoxia‑inducible factor-1α (HIF-1α), Fas and Bcl-2 were measured by western blotting. Significant inhibition of cell proliferation and induction of apoptosis were observed in FaDu and CAL-27&...
Source: International Journal of Molecular Medicine - Category: Molecular Biology Authors: Tags: Int J Mol Med Source Type: research
ConclusionThe PMF is simple, versatile and could be valued as a reconstructive alternative, with interesting visual qualities. It is a good therapeutic alternative tool for reconstruction of the buccal mucosa, especially for small- and medium-sized defects between 2 and 4  cm2 of the oral cavity.
Source: Oral and Maxillofacial Surgery - Category: ENT & OMF Source Type: research
Authors: Atallah I, Berta E, Coffre A, Villa J, Reyt E, Righini CA Abstract Glottic cancers discovered at an early stage (T1-T2) can be treated with either radiotherapy or surgery. The aim of our study is to analyse survival and functional results of supra-cricoid partial laryngectomy (SCPL) with crico-hyoido-epiglottopexy (CHEP) as surgical treatment for glottic carcinoma with anterior commissure involvement. We performed a retrospective study (1996-2013) which included patients who underwent SCPL-CHEP for glottic squamous cell carcinoma with involvement of the anterior commissure. Before surgery, all patients und...
Source: Acta Otorhinolaryngologica Italica - Category: ENT & OMF Tags: Acta Otorhinolaryngol Ital Source Type: research
The objective of the FDG PET/CT examination was twofold: (1) improve the radiotherapy target definition, and (2) identify the primary cancer. Before the FDG PET/CT, the patients had been through a standard workup consisting of CT of the neck and chest, examination with flexible endoscopy with patient awake, panendoscopy and examination under general anesthesia, tonsillectomy and sometimes blind sampling biopsies, and MRI (floor of the mouth). All FDG PET/CTs were performed applying a flat table, head support and fixation mask as part of the radiotherapy treatment planning. Diagnostic CT with contrast was an integrated part...
Source: European Archives of Oto-Rhino-Laryngology - Category: ENT & OMF Source Type: research
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