Functional Characterization of Potential PGPR Exhibiting Broad-spectrum Antifungal activity

This study describes the biocontrol potential ofrhizobacteria against a range of fungal phytopathogens. Out of 227 bacteria isolated from the rhizosphere of maize, rice, wheat, potato, sunflower and soybean crops cultivated in different agro-ecological regions of Pakistan, 48 exhibited>60% antifungal activity againstFusarium oxysporum, Fusarium moniliforme, Rhizoctonia solani, Colletotrichum gloeosporioides, Colletotrichum falcatum, Aspergillus niger, and Aspergillus flavus. The rhizobacteria inhibiting> 65% pathogen growth were selected for detailed molecular and in planta studies most of which were identified as Pseudomonas and Bacillus species based on 16S rRNA gene sequence analysis. Antifungal metabolites produced by these rhizobacteria analyzed through LCMS were identified as antibiotics (iturin, surfactins, fengycin, DAPG, Phenazine, etc.), cell wall degrading enzymes(protease, chitinase, and cellulase), plant growth promotion enzymes and hormones (indole-3-acetic acid, ACC-deaminase, phosphates, nitrogen fixation), N-acyl-homoserine lactones and siderophores. Thegrowth room experimentvalidated the potential of these bacteria asbiofertilizer and biopesticide agents. Of all, P. aeruginosa strain FB2 andB. subtilis strain RMB5showed significantly higher potential as antagonistic plant-beneficial bacteria effective against a range of fungal phytopathogens. Both these bacteria can be used to develop a dual-purpose bacterial inoculum as biopesticide and biofertilizer....
Source: Microbiological Research - Category: Infectious Diseases Source Type: research

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Authors: Sehgal IS, Dhooria S, Muthu V, Prasad KT, Agarwal R Abstract Introduction: Chronic pulmonary aspergillosis (CPA) is a chronic infection of the lung parenchyma caused by Aspergillus fumigatus complicating structural lung diseases. Of the different types of CPA, chronic cavitary pulmonary aspergillosis (CCPA) is the most common form. Untreated, most patients with CCPA will progress to chronic fibrosing pulmonary aspergillosis. The mainstay of treatment of CCPA is oral triazoles. However, many gaps exist in clinical decision-making about the agent of choice, the duration, and the assessment of treatment respo...
Source: Expert Review of Respiratory Medicine - Category: Respiratory Medicine Tags: Expert Rev Respir Med Source Type: research
Wed, 04/08/2020 - 14:13News blog
Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
ConclusionOur results showed that there is no difference in the FEV1 and forced vital capacity betweenAspergillus positive and negative patients in any asthma severity group.
Source: The Clinical Respiratory Journal - Category: Respiratory Medicine Authors: Tags: ORIGINAL ARTICLE Source Type: research
Authors: Kaur P, Kumar P, Randev S, Guglani V Abstract Allergic bronchopulmonary aspergillosis (ABPA) is an immunological disease complicating asthma or cystic fibrosis. An 8-year-old girl with no previous respiratory morbidity was diagnosed with ABPA on the basis of a raised eosinophil count and total and specific serum IgE levels combined with a positive skin-prick test for aspergillus and typical CT images of finger-in-glove hilar opacities and hyperattenuating mucous. She responded to treatment with itraconazole and corticosteroids and remains well. As far as we are aware, she is only the second child to be dia...
Source: Paediatrics and international child health - Category: Pediatrics Tags: Paediatr Int Child Health Source Type: research
Abstract The present study focused on phosphorus adsorption by novel fungal conidiophores biomass in aqueous solution. Fungal Conidiophores biomass was prepared from the fungal strains Aspergillus oryzae (YFK) and Fusarium oxysporum (YVS2). The functional groups and morphology of Conidiophores Biomass (CB) from these strains were characterized by FTIR and SEM. FTIR confirms the presence of alcohol, carboxylic acid, carbon dioxide, cyclic alkene, amine, alkene, fluoro compound, and halo compound groups. Batch mode study was carried out with two CB's such as Aspergillus oryzae CB (ACB) and Fusarium oxysporum CB (FCB...
Source: Environmental Science and Pollution Research International - Category: Environmental Health Authors: Tags: Environ Sci Pollut Res Int Source Type: research
The objective of this study was to investigate the effects of APAb test results on the clinical outcomes of patients with MAC-LD.
Source: Respiratory Medicine - Category: Respiratory Medicine Authors: Source Type: research
Abstract The current study was designed to isolate, identify and characterize a Bacillus sp. capable of producing protease and exhibiting antifungal activity. A highly potent bacterium capable of producing protease abundantly was isolated from the soil collected from the waste pit near Microbiology Laboratory of Birendra Multiple Campus, Bharatpur and later on identified as Lysinibacillus fusiformis strain SK on the basis of morphological, physiological, biochemical and 16S rDNA gene sequencing techniques. The strain SK showed 98.36% similarity with L. fusiformis strain NBRC 15717. Using R-programming statistical ...
Source: Current Microbiology - Category: Microbiology Authors: Tags: Curr Microbiol Source Type: research
Glucoamylase is one of the most industrially applied enzymes, produced by Aspergillus species, like Aspergillus niger. Compared to the traditional ways of process optimization, the metabolic engineering strategie...
Source: Microbial Cell Factories - Category: Microbiology Authors: Tags: Research Source Type: research
Super-resolution microscopy has evolved as a powerful method for subdiffraction-resolution fluorescence imaging of cells and cellular organelles, but requires sophisticated and expensive installations. Expansion microscopy (ExM), which is based on the physical expansion of the cellular structure of interest, provides a cheap alternative to bypass the diffraction limit and enable super-resolution imaging on a conventional fluorescence microscope. While ExM has shown impressive results for the magnified visualization of proteins and RNAs in cells and tissues, it has not yet been applied in fungi, mainly due to their complex ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
m AFJ Abstract Certain Aspergillus species such as Aspergillus flavus and A. parasiticus are well known for the formation of sclerotia. These developmental structures are thought to act as survival structures during adverse environmental conditions but are also a prerequisite for sexual reproduction. We previously described an A. niger mutant (scl-2) which formed sclerotium-like structures, suggesting a possible first stage of sexual development in this species. Several lines of evidence presented in this study support the previous conclusion that the sclerotium-like structures of scl-2 are indeed sclerotia. These...
Source: Fungal Genetics and Biology - Category: Biology Authors: Tags: Fungal Genet Biol Source Type: research
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