Inhibitory effects of Lactobacilli cocktail on HT-29 colon carcinoma cells growth and modulation of the Notch and Wnt/β-catenin signaling pathways

Publication date: Available online 2 November 2019Source: Microbial PathogenesisAuthor(s): Roya Ghanavati, Parisa Asadollahi, Mina Bahrololomi Shapourabadi, Shabnam Razavi, Malihe Talebi, Mahdi RohaniAbstractProbiotics could be considered as attractive candidates for preventing tumor growth through maintaining homeostasis. The aim of this study was to evaluate the inhibitory effect of a cocktail of five Lactobacillus species on human colorectal carcinoma cell line HT-29. The anti-proliferative and apoptotic effects of Lactobacilli cocktail were evaluated using MTT and flow cytometry tests, respectively. Quantitative real-time polymerase chain reaction (qPCR) was used to analyze the expression of several genes in the Notch (notch, hes1, msi1, and numb) and Wnt/β-catenin (CTNNB1 and CCND1) pathways, following the treatment of HT-29 cells with Lactobacilli cocktail. The treatment by Lactobacilli cocktail induced a significant anti-proliferative effect and late stage apoptosis among the cancer cells (p 
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research

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In this study, four different weight% of Punica granatum fruit peel extract were used as green stabilizers to synthesize IONPs followed by a series of characterization experiments and in vitro anticancer evaluation. Results revealed that all synthesized IONPs have high degree of crystallinity and purity. The optimum saturation magnetization, and hydrodynamic size were found to be ∼69 emu.g−1, and 26.52 nm, respectively. Morphological studies demonstrated that adding peel extract decreased size of NPs with the average particle size below 11 nm. Cytotoxicity assay showed that the IONPs were not react...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Integrin β4 (ITGB4) has been reported to be involved in carcinomas. Currently, ITGB4 has been characterized in colon cancer, however, its clinical significance is not very clear. In the present study, we utilized the large public datasets from NCBI Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases and collected clinical samples in our center to investigate the transcriptional expressions of ITGB4 in colon cancer, and then explored the associations of ITGB4 with clinicopathological features and overall survival. The statistical analyses suggested that ITGB4 mRNA expressions were up-regulated si...
Source: Journal of Cancer - Category: Cancer & Oncology Authors: Tags: Research Paper Source Type: research
ConclusionsSegmental left colectomy for splenic flexure carcinoma is associated with reasonably low morbidity and very good functional results. However, survival rates are low.
Source: Techniques in Coloproctology - Category: Surgery Source Type: research
In this study we were aiming to identify potent GLO-I inhibitors as potential candidates for the development of effective anticancer therapeutics using an integrated ligand- and structure-based drug design approach. A set of selective pharmacophore models was generated using an in-house tested set of flavonoids and used in virtual screening of Maybridge and Aldrich databases, collectively containing more than 64,000 compounds. Filtration of retained hits resulted in 362 compounds that were docked into the active site of the GLO-I enzyme. Then, the top 30% of docked compounds were visually inspected and 32 compounds were pu...
Source: Medicinal Chemistry Research - Category: Chemistry Source Type: research
Conclusions: These results indicated that CXCR4/TGF-β1 axis plays an important role in CRC liver metastasis through mediating HSCs differentiation into CAFs, providing preclinical evidences that blockade of the axis might be beneficial for anti-metastasis therapy in CRC. PMID: 31825725 [PubMed - as supplied by publisher]
Source: Cancer Biology and Therapy - Category: Cancer & Oncology Authors: Tags: Cancer Biol Ther Source Type: research
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Source: Journal of Gastrointestinal Cancer - Category: Cancer & Oncology Source Type: research
Publication date: Available online 6 December 2019Source: Saudi Journal of Biological SciencesAuthor(s): Muhammad Farooq, Nael Abutaha, Shahid Mahboob, Almohannad Baabbad, Nawaf D. Almoutiri, Mohammad Ahmed A.M. WadaanAbstractRecent trends in anticancer therapy is to use therapeutic agents which not only kill the cancer cell, but are less toxic to surrounding normal cells/tissue. One approach is to cut the nutrient supply to growing tumor cells, by blocking the formation of new blood vessels around the tumor. As the phytochemicals and botanical crude extracts have proven their efficacy as natural antiangiogenic agents with...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research
Mast cells constitutively express ß-catenin and expand in solid tumors such as colon and skin cancer. However, the role of ß-catenin signaling in mast cells and the cause or effect of mast cell expansion and tumor growth has yet to be established. In earlier studies we used mast cell depletion and protease staining approaches, to provide evidence for a causative role of mast cells in small bowel polyposis, and related specific phenotypes and distributions of tumor infiltrating mast cells to stages of tumor growth. Here we report that, stabilization of ß-catenin expands mast cells to promote high incidence...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: International Journal of Oncology - Category: Cancer & Oncology Authors: Tags: Int J Oncol Source Type: research
CONCLUSION: The proposed standardisation of laparoscopic right hemicolectomy with complete mesocolic excision accommodates the increased complexity of the right colon and structures it into well-defined steps with critical safety assessments, which may result in minimised intraoperative complications and increased patient safety and should improve training. PMID: 31791092 [PubMed - as supplied by publisher]
Source: Zentralblatt fur Chirurgie - Category: Surgery Authors: Tags: Zentralbl Chir Source Type: research
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