Silica Nano Supra-assembly for the targeted delivery of therapeutic cargo to overcome chemoresistance in cancer

Publication date: Available online 12 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Nanasaheb D. Thorat, Joanna Bauer, Syed A.M. Tofail, Victoria Gascón Pérez, Raghvendra A. Bohara, Hemraj M. YadavAbstractCancer cells become resistant over the period to chemotherapeutic drugs and pose a challenging impediment for oncologists in providing effective treatment. Nanomedicine allows to overcome chemoresistance and is the focus of our investigation. Silica nanostructures have been highlighted as an interesting drug delivery platform in vitro and in vivo applications. Here we show the validity of nanomedicine approach for targeted chemotherapeutic cargo delivery to overcome chemoresistance in cancer cells both in vitro and in vivo. For demonstrating the concept, we functionalised ˜100 nm long porous silica nanoparticles (˜20 nm diameter ordered pore structure) by conjugating anticancer drug, cytochrome c enzyme and dual-function anticancer aptamer AS1411 in single supra-assembled nanocargos. The supra-assembly on the porous silica nanostructure allows for a high loading of catalytic enzyme cytochrome c, anticancer drug and aptamer. The silica supra-assembly is characterized by transmission electron microscopy (TEM) and Brunauer-Emmett-Teller (BET) surface area analysis. Conjugation of cargoes has been monitored at each step by UV-Vis and Fluorescence spectroscopy. Finally, the constructed supra-assembled nanocarrier tested on chemoresistance colon canc...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research