Characterization of an Environmental Multidrug-Resistant Acinetobacter seifertii and Comparative Genomic Analysis Reveals Co-occurrence of Antimicrobial Resistance and Metal Tolerance Determinants

Acinetobacter calcoaceticus-Acinetobacter baumannii complex is considered one of the main causes of hospital-acquired infections. Acinetobacter seifertii was recently characterized within this complex and it has been described as an emergent pathogen associated with bacteremia. The emergence of multidrug-resistant (MDR) bacteria, including Acinetobacter sp., is considered a global public health threat and an environmental problem because MDR bacteria have been spreading from several sources. Therefore, this study aimed to characterize an environmental MDR A. seifertii isolate (SAb133) using whole genome sequencing and a comparative genomic analysis was performed with A. seifertii strains recovered from various sources. The SAb133 isolate was obtained from soil of a corn crop field and presented high MICs for antimicrobials and metals. The comparative genomic analyses revealed ANI values higher than 95% of relatedness with other A. seifertii strains than A. calcoaceticus-A. baumannii complex. Resistome and virulome analyses were also performed and showed different antimicrobial resistance determinants and metal tolerance genes as well as virulence genes related to A. baumannii known virulence genes. In addition, genomic islands, IS elements, plasmids and prophage-related sequences were detected. Comparative genomic analysis showed that MDR A. seifertii SAb133 had a high amount of determinants related to antimicrobial resistance and tolerance to metals, besides the presence of ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Semin Respir Crit Care Med 2019; 40: 454-464 DOI: 10.1055/s-0039-1693160Antibiotic resistance is recognized as a key determinant of outcome in patients with serious infections influencing empiric antibiotic practices especially for critically ill patients. Within the intensive care unit (ICU), nosocomial infections and increasingly community-onset infections are caused by multidrug-resistant bacteria. Escalating rates of antibiotic resistance adds substantially to the morbidity, mortality, and cost related to infections treated in the ICU. Both gram-positive organisms, such as methicillin-resistant Staphylococcus aureus an...
Source: Seminars in Respiratory and Critical Care Medicine - Category: Respiratory Medicine Authors: Tags: Review Article Source Type: research
ConclusionsThe effectiveness of COL alone in vitro and in vivo suggested that intravenous CMS will be an effective and available therapeutic strategy for UTI due to MDR gram-negative pathogens. In-depth in vitro tests demonstrated that COL combined with DOR could be an attractive option, especially when COL MIC of the pathogen is ≥ 1 μg/ml.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Acinetobacter baumannii is one of the most important nosocomial pathogens distributed worldwide. Due to its multidrug-resistance and the propensity for the epidemic spread, the World Health Organization includes this bacterium as a priority health issue for development of new antibiotics. The aims of this study were to investigate the antimicrobial resistance profile, the clonal relatedness, the virulence profiles, the innate host immune response and the clonal dissemination of A. baumannii in Hospital Civil de Guadalajara (HCG), Hospital Regional General Ignacio Zaragoza (HRGIZ) and Pediatric ward of the Hospital General ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
ConclusionThe prevalence of MDR-GNB in Iranian burn patients is very high. Thus, a comprehensive infectious control programme, a reduction in the use of antibiotic prophylaxis, and thorough information regarding antimicrobial resistance patterns is required.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
ConclusionThis is the first report of the genome sequence of an A. baumannii ST1928 clinical isolate carrying a novel class C β-lactamase gene from China. The genome sequence data can be used as a reference sequence for comparative studies and would facilitate further understanding of the antimicrobial resistance mechanisms of A. baumannii at the genomic level.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
[Latin American consensus to define, categorize, and report multidrug-resistant, extensively drug-resistant, or pandrug-resistant pathogensConsenso latino-americano para definição, categorização e notificação de patógenos multirresistentes, com resistência ampliada ou panresistentes]. Rev Panam Salud Publica. 2019;43:e65 Authors: Jiménez Pearson MA, Galas M, Corso A, Hormazábal JC, Duarte Valderrama C, Salgado Marcano N, Ramón-Pardo P, Melano RG Abstract This document presents a Latin American consensus to standardize definitio...
Source: Pan American Journal of Public Health - Category: International Medicine & Public Health Tags: Rev Panam Salud Publica Source Type: research
ConclusionThis is the first report of the genome sequence of A. baumannii ST1928 clinical isolate carrying a novel class C β-lactamase gene from China. The genome sequence data can be used as a reference sequence for comparative studies and would facilitate further understanding of the antibiotic resistance mechanisms of A. baumannii at the genomic level.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
ConclusionThe genome sequence of A. baumannii strain Ab174 can be used as a reference sequence for the new ST1688. This data will facilitate further understanding of genomic variation in A. baumannii isolates from different geographical regions.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
The lack of therapeutic options to treat infections caused by recalcitrant multidrug-resistant pathogens, especially Gram-negative bacteria, is apparent. Therefore, it is imperative to develop new strategies to address the problem of antimicrobial resistance. Repurposing non-antibiotic commercial drugs for antimicrobial therapy presents a viable option. We screened six anticancer drugs for their potential use in antimicrobial therapy. Here, we provide in vitro evidence that suggests feasibility to repurpose the anticancer drug mitomycin C against multidrug-resistant Gram-negative bacteria. We also demonstrated that mitomyc...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Multidrug-resistant (MDR) Acinetobacter baumannii infections are considered as emerging nosocomial infections particularly in patients hospitalized in intensive care units (ICUs). Therefore, reliable detection of...
Source: Annals of Clinical Microbiology and Antimicrobials - Category: Microbiology Authors: Tags: Research Source Type: research
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