Identification of differentially expressed genes and networks related to hepatic lipid dysfunction.

Identification of differentially expressed genes and networks related to hepatic lipid dysfunction. Toxicol Appl Pharmacol. 2019 Sep 11;:114757 Authors: Abedini JA, Handa S, Edwards S, Chorley B, El-Masri H Abstract A range of chemical exposures that resulted in the specific pathology of hepatic lipid dysfunction in rats were selected from DrugMatrix, a publicly available toxicogenomic database. Raw microarray data collected from these exposures were further analyzed using bioinformatic tools to generate a differentially expressed genes (DEGs) dataset associated with hepatic lipid dysfunction. Further analysis of the DEGs dataset resulted in 324 upregulated genes, and 275 genes that were down regulated. Meanwhile, 36 genes were either up regulated or down regulated in different chemical treatments. All identified genes were uploaded in the web application for Database for Annotation, Visualization and Integrated Discovery (DAVID) for gene ontology enrichments and to identify Kyoto Encyclopedia of Genes and Genome (KEGG) pathways. Some of the identified pathways included glycolysis/gluconeogenesis, steroid hormone biosynthesis, retinol metabolism, and metabolism of xenobiotics by cytochrome P450. The same DEGs dataset was also analyzed using Ingenuity Pathway Analysis (IPA) software. IPA identified several pathways including PXR/RXR activation, Aryl hydrocarbon receptor signaling, and xenobiotic metabolism signaling. Furthermore, the ...
Source: Toxicology and Applied Pharmacology - Category: Toxicology Authors: Tags: Toxicol Appl Pharmacol Source Type: research