GSE137317 Metformin-induced alterations in peripheral blood cell trancriptome of healthy individuals

Contributors : Monta Ustinova ; Ivars Silamikelis ; Ineta Kalnina ; Laura Ansone ; Vita Rovite ; Ilze Elbere ; Ilze Radovica-Spalvina ; Davids Fridmanis ; Jekaterina Aladyeva ; Ilze Konrade ; Valdis Pirags ; Janis KlovinsSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensMetformin is a commonly used antihyperglycaemic agent for the treatment of type 2 diabetes. Nevertheless, the exact mechanisms of action, underlying the various therapeutic effects of metformin, remain elusive. The goal of this study was to evaluate the alterations in longitudinal whole-blood transcriptome profiles of healthy individuals after a one-week metformin intervention in order to identify the novel molecular targets and further prompt the discovery of predictive biomarkers of metformin response. Next generation sequencing-based transcriptome analysis revealed four clusters of functionally related genes with a subject-specific differential expression even after a single dose of metformin: ribosomal genes and their pseudogenes (e.g., RNA5-8SN1, RNA5-8SN5, RNY4P10), small nuclear RNAs (e.g., SNORA20, SCARNA5, SNORA80E), genes relevant to insulin production (HNF1B, HNF1A, HNF4A, GCK, INS, NEUROD1, PAX4, PDX1, ABCC8, KCNJ11) and cholesterol homeostasis (APOB, LDLR, PCSK9). After accounting for subject-specific expression, the enrichment of pathways associated with immune responses, such as intestinal immune network for IgA production and cytokine-cytokine r...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research