Fast cyclical-decellularized trachea as a natural 3D scaffold for organ engineering

Publication date: December 2019Source: Materials Science and Engineering: C, Volume 105Author(s): David M. Giraldo-Gomez, Sandra Julieta García-López, Lenin Tamay-de-Dios, Roberto Sánchez-Sánchez, Jaime Villalba-Caloca, Avelina Sotres-Vega, María Luisa Del Prado-Audelo, Karla K. Gómez-Lizárraga, David Garciadiego-Cázares, María Cristina Piña-BarbaAbstractCommonly reported decellularization protocols for trachea may take up from several weeks to months in order to remove the cellular materials. Two years ago, we significantly reduced the time of decellularization trachea process using trypsin. Despite the positive outcome, the protocol was useful to produce 5 cm graft length, an unsuitable length graft for most patients with tracheal disorders. In this work we improved the decellularization procedure for longer sections up to 10 cm without considerable extension in the necessary time process (2 weeks). Herein, for the first time, we completely describe and characterize the process for pig tracheal bioactive scaffolds. Histological and molecular biology analysis demonstrated effective removal of cellular components and nuclear material, which was also confirmed by the Immunohistochemical (IHC) analysis of the major histocompatibility complexes (MHCs) and DNA stain by 4′-6-diamidino-2-phenylindole (DAPI). The images and data obtained from scanning electron microscopy (SEM) and thermal analysis showed conservation of the hierarchical structures of the tracheal e...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research