Prospects of the Application of Buccal Epithelium for Noninvasive Diagnosis of Coronary Heart Disease in People of Different Ages

AbstractCardiovascular diseases caused by atherosclerosis are one of the most important social and economic problems in many countries of the world due to the high morbidity and mortality rate of the working population. An immunological theory of atherosclerosis and coronary heart disease (CHD) has been actively developed of late, and markers of inflammation characterizing immuno- and atherogenesis have been sought. The buccal epithelium (BE) can be used as biological material for in vivo molecular-cellular studies, allowing CHD diagnosis via inflammation markers. The purpose of the work was a comparative study of the expression ofIL-1 β,IL-6,IL-10,MCP-1, andGDF-15 in BE in patients of different ages with CHD and without cardiovascular disease. The BE material in healthy donors and patients with second-stage CHD was divided into groups according to the age classification of the WHO: the first group included middle-aged people (45 –59 years), and the second included elderly people (60–74 years). Control material was obtained from people of middle and old age without cardiovascular disease. According to the immunocytochemical study, the area ofIL-1 β expression in BE is three times higher in middle-aged CHD patients and 4.4 times higher in elderly people as compared to healthy individuals of the same age group. The area ofIL-6 expression in middle-aged and elderly CHD patients was 7.9 and 7.4 times higher, respectively, than in the control group. In middle-aged and elderl...
Source: Advances in Gerontology - Category: Geriatrics Source Type: research