Direct electrochemical determination of methotrexate using functionalized carbon nanotube paste electrode as biosensor for in-vitro analysis of urine and dilute serum samples

Publication date: July 2019Source: Microchemical Journal, Volume 148Author(s): Shekher Kummari, V. Sunil Kumar, M. Satyanarayana, K. Vengatajalabathy GobiAbstractChemotherapy is a widely used effective method for the treatment of cancer in humans. Severity of dose levels and treatment duration necessitate frequent continuous monitoring of active drug concentration in human serum and urine. Development of an electrochemical biosensor for direct determination of methotrexate (MTX), a chemotherapy agent, from physiological fluids has been investigated. Functionalized multi-walled carbon nanotube paste electrodes (f-CNTPE) have been fabricated for simple, cost-effective and reusable electrochemical sensors. Scanning electron microscopy studies revealed that the fabricated electrode surface is comprised of nano-streaks of independent segregated CNTs with high porosity. Cyclic voltammetric analysis showed that f-CNTPE had an enhanced electrocatalytic activity with sensitivity as much as three times and less over potentials by 70 mV compared with a conventional carbon paste electrode. Under optimized experimental parameters, differential pulse voltammograms (DPVs) exhibited a gradual increase in peak current with concentration, and the plot of peak current against MTX concentration was linear in the range of 0.4–5.5 μM. Square-wave voltammetry analysis of methotrexate exhibited a linear determination range from 0.01 to 1.5 μM, and the low-detection-limit was 2.9 ...
Source: Microchemical Journal - Category: Chemistry Source Type: research

Related Links:

ConclusionPrimary adenocarcinoma of the jaw is extremely rare, except for 2–3% central mucoepidermoid carcinomas. The possibility of a metastatic tumor should be a consideration when encountering unusual histomorphology of an adenocarcinoma in the jaw bone.
Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology - Category: ENT & OMF Source Type: research
CONCLUSIONWe report a rare case of hepatic anisakiasis which initially presented as suspected metastatic cancer. Hepatic anisakiasis should be considered in the differential diagnosis of low density liver tumours less than 20 mm, where the patient’s diet includes raw fish.
Source: International Journal of Surgery Case Reports - Category: Surgery Source Type: research
Publication date: October 2019Source: Materials Science and Engineering: C, Volume 103Author(s): Mohadeseh Hashemi, Javad Mohammadi, Meisam Omidi, Hugh D.C. Smyth, Bharadwaj Muralidharan, Thomas E. Milner, Amir Yadegari, Davoud Ahmadvand, Mohammad Shalbaf, Lobat TayebiAbstractThis paper describes the design of stimuli-sensitive theranostic nanoparticles, composed of reduced graphene oxide (rGO) self-assembled on thermosensitive liposomes encapsulated doxorubicin (DOX) and carbon quantum dot (CQD) (CQD-DOX-rGO-Tlip). The rGO-Tlip particles have been observed to be flower-shaped objects. The thermoresponsive and theranostic ...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Conclusion: Results from the current observational study found similar point estimates for 5-year survival and of relative risks as previous studies. However, the results based on real world evidence had larger statistical variability, resulting in a non-statistically significant effect of NAC on survival. Future studies with detailed validated data can be used to further investigate the effect of NAC in narrower patient groups. PMID: 31174452 [PubMed - as supplied by publisher]
Source: Scandinavian Journal of Urology - Category: Urology & Nephrology Tags: Scand J Urol Source Type: research
Conclusion: These findings underline the need for proper patient information in order to get a uniform treatment strategy between centres. PMID: 31174449 [PubMed - as supplied by publisher]
Source: Scandinavian Journal of Urology - Category: Urology & Nephrology Tags: Scand J Urol Source Type: research
This study aims to elucidate whether the vertebral accumulation of18F-FLT could estimate severe HT during platinum-doublet chemotherapy.MethodsIn this Institutional Review Board –approved retrospective study, 50 patients with primary lung cancer underwent18F-FLT PET scan before platinum-doublet chemotherapy. We evaluated the standardized uptake value, total vertebral proliferation (TVP), and TVP/body surface area (TVP/BSA) of the vertebral body (Th4, Th8, Th12, and L4), and then the associations between those parameters and frequency of severe HT during platinum-doublet chemotherapy were assessed.ResultsSevere HT (gr...
Source: European Radiology - Category: Radiology Source Type: research
ConclusionTumors responding to first-line chemotherapy also responded better to subsequent lines, whereas PDfl mCRCs remained refractory, which may explain the better survival associated with ETSfl.Key Points• Early shrinking tumors under first-line chemotherapy responded better to subsequent lines, maintaining low tumor loads, potentially explaining the link between early tumor shrinkage and overall survival of metastatic colorectal cancer (mCRC) patients.• mCRCs progressing under first-line chemotherapy remained refractory to other therapies and their tumor loads increased rapidly.• Even outside a clinical...
Source: European Radiology - Category: Radiology Source Type: research
This study quantifies the disturbing trend that, despite the expansion in the number of patients eligible for expensive and potentially toxic ICIs [immune checkpoint inhibitors], the ratio of those benefiting is decreasing,” wrote Dr. Daniel V.T. Catenacci, co-author of an editorial that accompanied the study in JAMA Network. “Observations in this article are sobering and remind us to keep expectations realistic.” Both Catenacci and Haslem agreed that the study should serve as a reminder to patients, physicians and policy makers to have more realistic discussions about the use of, and expectations of, the...
Source: Asbestos and Mesothelioma News - Category: Environmental Health Authors: Source Type: news
CONCLUSION: The adoption of the GHSG risk model at the time of recurrence/progression is a useful prognostic tool to select patients with Hodgkin lymphoma for consolidative involved-field radiotherapy after autologous stem cell transplantation. PMID: 31178272 [PubMed - as supplied by publisher]
Source: Cancer Radiotherapie - Category: Cancer & Oncology Authors: Tags: Cancer Radiother Source Type: research
Authors: Petzold T, Thienel M, Lüsebrink E, Massberg S Abstract A patient with a history of colon cancer was admitted to our emergency department due to progressive dyspnea, chest pain, and swelling of the upper limbs and face. A central venous port device had been implanted into the right subclavian vein for administration of systemic chemotherapy several months prior. Thoracic computed tomography scan revealed a 2.8 cm-long thrombus arising from the tip of the venous catheter, which completely occluded the superior vena cava in close proximity to the right atrium. PMID: 31158815 [PubMed - in process]
Source: The Journal of Invasive Cardiology - Category: Cardiology Tags: J Invasive Cardiol Source Type: research
More News: Cancer | Cancer & Oncology | Chemistry | Chemotherapy | Methotrexate | Nanotechnology | PET Scan | Study | Vitamin C