Chemical characterization, antioxidant properties, cholinesterase inhibitory and anti-amyloidogenic activities of sulfated polysaccharides from some seaweeds

Publication date: Available online 11 March 2019Source: Bioactive Carbohydrates and Dietary FibreAuthor(s): Tosin A. Olasehinde, Leonard V. Mabinya, Ademola A. Olaniran, Anthony I. OkohAbstractSulfated polysaccharides were extracted from Ecklonia maxima (PKPM), Gelidium pristoides (PMNP) and Ulva rigida (PURL) and characterized using scanning electron microscope with energy dispersing x-ray (SEM-EDX), Fourier transform infrared spectroscopy (FTIR) and Gas Chromatography-coupled with mass spectrometry (GC-MS). The radical scavenging activity, metal chelating effects, enzyme inhibitory potentials and anti-amyloidogenic activities of the sulfated polysaccharide samples were also evaluated. The elemental analysis and FTIR results confirmed the presence of sulfate groups in the polysaccharides. Furthermore, GC-MS analysis revealed the presence of glucose, galactose, fucose, arabinose and xylose as the main monosaccharides present in the samples. PKPM, PMNP and PURL exhibited radical scavenging activities against DPPH, ABTS and OH radicals and were able to chelate Fe2+. The sulfated polysaccharides also showed inhibitory effects on acetylcholinesterase, butyrylcholinesterase and β-secretase activities. PKPM showed higher cholinesterase and β-secretase inhibitory activities than PURL and PMNP. Thioflavin-T assay revealed that incubation of β-amyloid (Aβ1-42) peptide without the polysaccharides showed high fluorescence intensity compared to Aβ1-42 treated with PMNP, PKPM and PUR...
Source: Bioactive Carbohydrates and Dietary Fibre - Category: Food Science Source Type: research