RNA-Seq and protein mass spectrometry in microdissected kidney tubules reveal signaling processes initiating lithium-induced nephrogenic diabetes insipidus

Lithium salts, used for treating bipolar disorder, frequently induce nephrogenic diabetes insipidus (NDI) thereby limiting therapeutic success. NDI is associated with loss of expression of the gene coding for the molecular water channel, aquaporin-2, in the renal collecting duct (CD). Here, we use systems biology methods in a well-established rat model of lithium-induced NDI to identify signaling pathways activated at the onset of polyuria. Using single-tubule RNA-Seq, full transcriptomes were determined in microdissected cortical collecting ducts of rats after 72 hours without or with initiation of lithium chloride administration.

Source: Kidney International - March 3, 2019 Category: Urology & Nephrology Authors: Chih-Chien Sung, Lihe Chen, Kavee Limbutara, Hyun Jun Jung, Gabrielle G. Gilmer, Chin-Rang Yang, Shih-Hua Lin, Sookkasem Khositseth, Chung-Lin Chou, Mark A. Knepper Tags: Basic Research Source Type: research