Antimalarial Activity of Piperine.

Antimalarial Activity of Piperine. J Trop Med. 2018;2018:9486905 Authors: Thiengsusuk A, Muhamad P, Chaijaroenkul W, Na-Bangchang K Abstract Malaria remains a public health problem in tropical and subtropical regions. Resistance of Plasmodium falciparum to artemisinins in Southeast Asia is a great concern for disease control and research on discovery and development of new alternative antimalarial drugs is urgently required. In a previous study, the fruit of Piper chaba Hunt. was demonstrated to exhibit promising antimalarial activity against the asexual stage of 3D7 (chloroquine-sensitive) and K1 (chloroquine-resistant) P. falciparum clones. The aim of the present study was to further investigate the antimalarial activity of piperine, the major isolated constituent of Piper chaba Hunt. fruits against both P. falciparum clones. The antimalarial activity was determined using SYBR green-I-based assay and morphological change was observed under the light microscope with Giemsa staining. The median IC50 (concentration that inhibits parasite growth by 50%) values of piperine against 3D7 and K1 P. falciparum were 111.5 and 59 μM, respectively. A marked change in parasite morphology was observed within 48 hours of piperine exposure. Results of real-time PCR showed no effect of piperine on modulating the expression of the three genes associated with antimalarial drug resistance in P. falciparum, i.e., pfcrt, pfmdr1, and pfmrp1. Piperine could be a promising ca...
Source: Journal of Tropical Medicine - Category: Tropical Medicine Tags: J Trop Med Source Type: research

Related Links:

The low bioavailability of nitric oxide (NO) and its precursor, arginine, contributes to the microvascular pathophysiology of severe falciparum malaria. To better characterize the mechanisms underlying hypoargininemia in severe malaria, we measured the plasma concentrations of amino acids involved in de novo arginine synthesis in children with uncomplicated falciparum malaria (UM; n = 61), children with cerebral falciparum malaria (CM; n = 45), and healthy children (HC; n = 109). We also administered primed infusions of l-arginine uniformly labeled with 13C6 and 15N4 to 8 children with severe falciparum malaria (SM; age ra...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Fungal and Parasitic Infections Source Type: research
Plasmodium falciparum erythrocyte-binding antigen 140 (EBA-140) plays a role in tight junction formation during parasite invasion of red blood cells and is a potential vaccine candidate for malaria. Individuals in areas where malaria is endemic possess EBA-140-specific antibodies, and individuals with high antibody titers to this protein have a lower rate of reinfection by parasites. The red blood cell binding segment of EBA-140 is comprised of two Duffy-binding-like domains, called F1 and F2, that together create region II. The sialic acid-binding pocket of F1 is essential for binding, whereas the sialic acid-binding pock...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Microbial Immunity and Vaccines Source Type: research
Publication date: Available online 22 March 2019Source: The LancetAuthor(s): Richard Kajubi, Teddy Ochieng, Abel Kakuru, Prasanna Jagannathan, Miriam Nakalembe, Theodore Ruel, Bishop Opira, Harriet Ochokoru, John Ategeka, Patience Nayebare, Tamara D Clark, Diane V Havlir, Moses R Kamya, Grant DorseySummaryBackgroundIntermittent treatment with sulfadoxine–pyrimethamine, recommended for prevention of malaria in pregnant women throughout sub-Saharan Africa, is threatened by parasite resistance. We assessed the efficacy and safety of intermittent preventive treatment with dihydroartemisinin–piperaquine as an altern...
Source: The Lancet - Category: General Medicine Source Type: research
This article is the first systematic review of the effects of biosynthesized nanoparticles on both malaria parasites (Plasmodium spp.) and relevant vectors.Graphical Abstract
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research
In this study, we investigated the prevalence of antifolate-resistance mutations inPlasmodium falciparum that are associated with SP resistance in HIV-infected individuals on antiretroviral treatment randomized to discontinue (STOP-CTX), or continue (CTX) cotrimoxazole in Western Kenya. DesignSamples were obtained from an unblinded, non-inferiority randomized controlled trial where participants were recruited on a rolling basis for the first six months of the study, then followed-up for 12 months with samples collected at enrollment, quarterly, and during sick visits. MethodPlasmodium DNA was extracted from blood specimens...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
CONCLUSION: Strategies to improve currently available diagnostic techniques, and studies dealing with vector control procedures and other operational tools and approaches are needed for better understanding of this health problem. PMID: 30882372 [PubMed - in process]
Source: Infezioni in Medicina - Category: Infectious Diseases Tags: Infez Med Source Type: research
In conclusion, such multifunctional ART-HEP-NCPs with higher ART loading and extended half-life could be a promising platform for targeted antimalarial drug delivery.Graphical abstractChemical structure of ART-conjugated heparin, its self-assembly and delivery to iRBCs.
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
Condition:   Malaria,Falciparum Intervention:   Biological: Plasmodium falciparum malaria parasite Sponsor:   U.S. Army Medical Research and Materiel Command Not yet recruiting
Source: - Category: Research Source Type: clinical trials
Authors: Ohno T, Miyasaka Y, Kuga M, Ushida K, Matsushima M, Kawabe T, Kikkawa Y, Mizuno M, Takahashi M Abstract Malaria is caused by Plasmodium parasites and is one of the most life-threatening infectious diseases in humans. Infection can result in severe complications such as cerebral malaria, acute lung injury/acute respiratory distress syndrome, and acute renal injury. These complications are mainly caused by P. falciparum infection and are major causes of death associated with malaria. There are a few species of rodent-infective malaria parasites, and mice infected with such parasites are now widely used for s...
Source: Experimental Animals - Category: Research Tags: Exp Anim Source Type: research
The malarial parasite Plasmodium falciparum is an auxotroph for purines, which are required for nucleic acid synthesis during the intra-erythrocytic developmental cycle (IDC) of the parasite. The capabilities of ...
Source: Malaria Journal - Category: Infectious Diseases Authors: Tags: Research Source Type: research
More News: Fruit | Genetics | International Medicine & Public Health | Malaria | Parasitic Diseases | Parasitology | Study | Tropical Medicine