Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro

Publication date: Available online 18 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Karin Luize Mazur, Paulo Emílio Feuser, Alexsandra Valério, Arthur Poester Cordeiro, Camila Indiani de Oliveira, João Paulo Assolini, Wander Rogério Pavanelli, Claudia Sayer, Pedro H.H. AraújoAbstractLeishmaniasis is considered a neglected tropical disease that represents a Public Health problem due to its high incidence. In the search of new alternatives for Leishmaniasis treatment diethyldithiocarbamate (DETC) has shown an excellent leishmanicidal activity and the incorporation into drug carrier systems, such as solid lipid nanoparticles (SLNs), is very promising. In the present work DETC loaded in beeswax nanoparticles containing copaiba oil were obtained by the double emulsion/melt technique. The nanoparticles were characterized and leishmanicidal activity against L. amazonensis promastigotes forms and cytotoxicity in murine macrophages were evaluated. SLNs presented size below 200 nm, spherical morphology, negative charge surface, high encapsulation efficiency, above 80%, and excellent stability. Moreover, Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) analyses were performed to evaluate the chemical structure and possible interactions between DETC and SLNs. SLNs provided a protection for DETC, decreasing its cytotoxic effects in macrophages, which led to an improvement in the selectivity against the parasites, whi...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research