A new active nanocomposite film based on PLA/ZnO nanoparticle/essential oils for the preservation of refrigerated Otolithes ruber fillets

Publication date: March 2019Source: Food Packaging and Shelf Life, Volume 19Author(s): Mojtaba Heydari-Majd, Babak Ghanbarzadeh, Mostafa Shahidi-Noghabi, Mohammad Ali Najafi, Mohammadyar HosseiniAbstractIn the current study, the active packaging films based on poly lactic acid (PLA) containing 1.5% w/w zinc oxide (ZnO) nanoparticles and varying concentrations (0.5, 1, 1.5% w/w) of Zataria multiflora Boiss. essential oil (ZEO) and Menthe piperita L. essential oil (MEO) were produced. The efficacies of the resulted active films on shelf life extension of Otolithes ruber fish during 16 days of refrigerated storage (4 ± 1 °C) were evaluated. The antibacterial activity of the films was tested against five common food-borne pathogens (Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, Bacillus cereus and Staphylococcus aureus) by disc diffusion method. The fillet samples were analyzed periodically for microbiological (aerobic count, Enterobacteriaceae count, Pseudomonas spp., Lactic acid bacteria (LAB) count and H2S-producing bacteria) and chemical (TBARS and TVB-N). PLA/ZnO/ZEO and PLA/ZnO/MEO films showed enhanced antibacterial (691 and 513.33 mm2, repectively, against S. aureus) and antioxidant (69.14 and 49.08%, repectively) activity than control. Based on the microbial count, the active films significantly increased the shelf life of fillets from 8 to 16 days. The lowest TBARS (0.8 mg MA/kg muscle) and TVB-N (21.23 mg/100 g muccle) values were obtai...
Source: Food Packaging and Shelf Life - Category: Food Science Source Type: research