Anticancer effects of alloxanthoxyletin and fatty acids esters – In vitro study on cancer HTB-140 and A549 cells

Publication date: February 2019Source: Biomedicine & Pharmacotherapy, Volume 110Author(s): Michał Jóźwiak, Marta Struga, Piotr Roszkowski, Agnieszka Filipek, Grażyna Nowicka, Wioletta OlejarzAbstractAlloxanthoxyletin, a natural occurring pyranocoumarin isolated from a number of plant sources, such as family of Rutaceae, and its synthetic derivatives show cytotoxic and antitumor activities. In the present study new eleven esters of alloxanthoxyletin and fatty acids were synthesized and evaluated for their anticancer toxicity. The structures of the compounds were confirmed by Proton Nuclear Magnetic Resonance (1H NMR), Carbon-13 Nuclear Magnetic Resonance (13C NMR) and High Resolution Mass Spectrometry (HRMS) analyses.For all compounds 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cytotoxic effect on human melanoma cells (HTB-140), human epithelial lung carcinoma cells (A549) and human keratinocyte line (HaCaT). For the most active compounds (8–11) lactate dehydrogenase (LDH) assay to assess the level of cell damage as well as migration inhibition assay were performed. To explain the basic mechanism of cell death induction, the effect of derivatives 8–11 on early and late apoptosis in Annexin V-FITC/7-AAD flow cytometry analysis was investigated.The results indicate that human melanoma cells (HTB-140) and human epithelial lung carcinoma cells (A549) were more sensitive to new alloxanthoxyletin derivatives exposure comp...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research