Enhanced expression of the Epithelial Sodium Channel in neutrophils from hypertensive patients

Publication date: Available online 10 November 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Erika Reus-Chavarría, Ivette Martínez-Vieyra, Cristina Salinas-Nolasco, Araceli Evangelina Chávez-Piña, Juan Vicente Méndez-Méndez, Edgar Oliver López-Villegas, Alejandro Sosa-Peinado, Doris CerecedoAbstractHypertension (HTN), i.e. abnormally high blood pressure, is a major risk factor for heart attack, stroke, and kidney failure. The Epithelial Sodium Channel (ENaC), one of the main transporters regulates blood pressure by tightly controlling the sodium reabsorption along the nephron. Recently, we have shown an α-ENaC overexpression in platelets from hypertensive patients compared to platelets from normotensive subjects, suggesting it makes a contribution to the activation state of platelets and the physiopathology of hypertension. However, the involvement of the α-ENaC localized in neutrophils to this disease remains unknown. Neutrophils are the first leukocytes to be recruited to an inflammatory site and are equipped with a strong ability to eliminate intra- or extracellular pathogens using reactive oxygen species or antibacterial proteins contained in their granules.Using the Western blotting (Wb), flow cytometry, and qRT-PCR approaches; we determined α-ENaC neutrophil overexpression at the protein and messenger RNA (mRNA) levels. By confocal and cytometry analysis, we determined the α-ENaC distribution and the heterogeneity of HTN neutrophils...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - Category: Biochemistry Source Type: research