A highly multiplexed broad pathogen detection assay for infectious disease diagnostics
by Jeffrey W. Koehler, Christina E. Douglas, Timothy D. Minogue Rapid pathogen identification during an acute febrile illness is a critical first step for providing appropriate clinical care and patient isolation. Primary screening using sensitive and specific assays, such as real-time PCR and ELISAs, can rapidly test for known circulating infectious diseases. If the initial testing is negative, potentially due to a lack of developed diagnostic assays or an incomplete understanding of the pathogens circulating within a geographic region, additional testing would be required including highly multiplexed assays and metagenomic next generation sequencing. T o bridge the gap between rapid point of care diagnostics and sequencing, we developed a highly multiplexed assay designed to detect 164 different viruses, bacteria, and parasites using the NanoString nCounter platform. Included in this assay were high consequence pathogens such as Ebola virus, highl y endemic organisms including severalPlasmodium species, and a large number of less prevalent pathogens to ensure a broad coverage of potential human pathogens. Evaluation of this panel resulted in positive detection of 113 (encompassing 98 different human pathogen types) of the 126 organisms available to us including the medically important Ebola virus, Lassa virus, dengue virus serotypes 1 –4, Chikungunya virus, yellow fever virus, andPlasmodium falciparum. Overall, this assay could improve infectious disease diagnostics ...
Analyst, 2019, Accepted Manuscript DOI: 10.1039/C9AN01631G, PaperEmerson A Fonseca, Lucas Lafet á, Renan Cunha, Hudson Miranda, João Campos, Helton Medeiros, Marco Aurelio Romano-Silva, Raigna Silva, Alexandre Barbosa, Rafael Vieira, L Malard, Ado Jorio Global prevalence of Alzheimer ’s disease (AD) points to endemic levels, especially considering the increase on average life expectancy worldwide. AD diagnosis based on early biomarkers, in addition to a better... The content of this RSS Feed (c) The Royal Society of Chemistry
This week's case was donated by Blaine Mathison and Marc Couturier. The following forms were seen on peripheral blood smears. No travel history is available at this time. How would you recommend reporting out this case? Are there any additional studies you would recommend?
[Vanguard] Health authorities in the Democratic Republic of Congo will introduce a Johnson&Johnson Ebola vaccine in November in the country's eastern provinces, to counter the current outbreak, they said.
The objectives for entrepreneurship include an alliance to enhance the development and productivity of rural Colombia, promoting a productive transformation with more innovation, increased labour and business formalization, and better public goods and services. Framed within this overall response, the Ministry of Agriculture, in collaboration with the International Fund for Agricultural Development (IFAD), has launched a new program, “El Campo Emprende” [Rural areas are enterprising], that benefits small producers, indigenous peoples, Afro-descendant communities, female heads of household, young people, and in ...
[Observer] -- Stakeholders grade project successful
[Deutsche Welle] Many in the Democratic Republic of Congo have been waiting for this moment: A new vaccine to battle the Ebola outbreak is about to be released. The vaccine could reach broader parts of the population than before.
Contributors : Kim Eun-Min ; Yong Tai-SoonSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensA total of 1,301 differentially expressed genes were identified: 521 upregulated and 780 downregulated. Furthermore, p21 and PAI levels decreased, which coincided with decreases in E-cadherin and increases in fibronectin expression, in cells stimulated with Clonorchis sinensis excretory/secretory products (ESPs) and NDMA. Cancer-related proteins, such as EGFR, K-ras, and PCNA, were significantly upregulated in cells exposed to C. sinensis ESPs and NDMA. Our results showed that C. sinensis ESPs an...
Journal of Medicinal ChemistryDOI: 10.1021/acs.jmedchem.9b01099
Nature, Published online: 14 October 2019; doi:10.1038/d41586-019-03103-4The deadly virus is one of five that have been brought to a secure laboratory.
We examined 438 fresh skulls of pine marten (Martes martes) from Germany to check for the presence of the cranial trematodeTroglotrema acutum. The parasite was detected in the frontal sinuses of one 3-year-old road-killed male from the Rhoen Mountains (Thuringia). We detected 19 adultT. acutum and 34 eggs. The infested pine martens showed no deformations or lesions of the skull. This is the first time that specimens of the trematode were recovered from a pine marten from Germany, but the low prevalence of 0.23% confirms that the pine marten is only a rare host ofT. acutum.