Synthesis, characterization and unravelling the binding interaction of new bioactive 4-hydroxycoumarin derivative with calf thymus DNA: Insights from spectroscopic and theoretical aspect

Publication date: Available online 13 October 2018Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Abhijit Mukherjee, Suvranil Ghosh, Rudraditya Sarkar, Satyajit Samanta, Sudipto Ghosh, Mahadeb Pal, Adinath Majee, Sukanta Kumar Sen, Bula SinghAbstractIn the progress of small molecule drug candidates, 4-hydroxycoumarin based compounds bearing a crucial place as potent antibiotic agents with appreciable safety in drug invention. Being synthetically and easily obtainable, 4-hydroxycoumarin related compounds with planar structure have been promoted predominantly as DNA targeting agent. Nevertheless, here we elucidate the synthesis, characterization and theoretical study of bio-active small molecule 4-hydroxy-3,4′-bichromenyl-2,2′-dione (4HBD). Then we have illuminated the binding interactions of 4HBD with calf thymus DNA (ctDNA), which is particularly designed for biological application. Extensive investigations of the binding of 4HBD with ctDNA are provided by utilizing multi-spectroscopic and molecular docking approaches, including UV–vis absorbance, steady-state, time-resolved fluorescence spectroscopy and circular dichroism study. The calculated binding and quenching constant value from quantitative data analysis of absorption and emission spectroscopy shows that 4HBD binds to the ctDNA groove. Further confirmation of the same is found by comparative displacement and iodide quenching studies. Negative enthalpy, negative free energy and positive en...

Source: Journal of Photochemistry and Photobiology B: Biology - October 13, 2018 Category: Speech-Language Pathology Source Type: research