Overexpression of the Thyroid Hormone-Responsive (THRSP) Gene in the Striatum Leads to the Development of Inattentive-like Phenotype in Mice

Publication date: Available online 21 August 2018Source: NeuroscienceAuthor(s): Raly James Perez Custodio, Chrislean Jun Botanas, June Bryan de la Pena, Irene Joy dela Pena, Mikyung Kim, Leandro Val Sayson, Arvie Abiero, Zae Young Ryoo, Bung-Nyun Kim, Hee Jin Kim, Jae Hoon CheongAbstractAttention-deficit/hyperactivity disorder (ADHD) is a neurodevelopmental disorder that affects 8–12% of children globally. Factor analyses have divided ADHD symptoms into two domains: inattention and a combination of hyperactivity and impulsivity. The identification of domain-specific genetic risk variants may help uncover potential genetic mechanisms underlying ADHD. We have previously identified that thyroid hormone-responsive (THRSP) gene expression is upregulated in spontaneously hypertensive rats (SHR/NCrl) and Wistar-Kyoto (WKY/NCrl) rats which exhibited inattention behavior. Thus, we established a line of THRSP overexpressing (OE) mice and assessed their behavior through an array of behavioral tests. The gene and protein overexpression of THRSP in the striatum (STR) was confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting. The THRSP OE mice exhibited inattention in the novel-object recognition and Y-maze test, but not hyperactivity and impulsivity in the open-field test and cliff-avoidance and delay-discounting task, respectively. We have also found that expression of dopamine-related genes (dopamine transporter, tyrosine hydroxylase, and dopamin...
Source: Neuroscience - Category: Neuroscience Source Type: research