Antioxidant and anti-inflammatory properties of marmelosin from Bael (Aegle marmelos L.); Inhibition of TNF-α mediated inflammatory/tumor markers

Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Hasitha Pynam, Shylaja M. DharmeshAbstractOxidative stress and inflammation are important critical factors that are implicated in almost all life style disorders such as diabetes, cardiovascular disease, ulcer and cancer. Current study aimed at isolation and characterization of a furanocoumarin from Bael (Aegle marmelos L.) fruit that can modulate both oxidative stress and inflammation effectively. Ethyl acetate extract of Bael fruit (EAFB) was subjected to HPLC for identification, purified and characterized using FTIR, NMR and ESI-MS analysis. Predominant peak of EAFB at RT 12.54 min on HPLC was identified as marmelosin with molecular weight of m/z ∼ 271.2. Marmelosin was evaluated for antioxidant, antiproliferative, apoptotic, cancer (Tyrosinase & Galectin-3) and immunomodulatory (NO, TNF-α) potentials employing standard assay systems. Marmelosin possessed potent antioxidant activity with IC50 of ∼ 15.4 ± 0.32 μM as opposed to standard - gallic acid (IC50 1.1 ± 0.08 μM), antiproliferative activity with IC50 of ∼ 6.24 ± 0.16 μM as opposed to deferoxamine (∼10.8 ± 0.28 μM) and protected cells against cellular/DNA damage. Anti-inflammatory property was evident with significant reduction in the release of NO (∼3.9 fold) and TNF-α (∼3.4 fold), a pro-inflammatory cytokine, in addition to the inhibition of NFκB (∼2.7 fold), a transcription factor in ...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research