New mouse model of pulmonary hypertension induced by respiratory syncytial virus bronchiolitis.

New mouse model of pulmonary hypertension induced by respiratory syncytial virus bronchiolitis. Am J Physiol Heart Circ Physiol. 2018 Jun 15;: Authors: Kimura D, Saravia J, Jaligama S, McNamara I, Vu LD, Sullivan RD, Mancarella S, You D, Cormier SA Abstract Pulmonary hypertension (PH) has been observed in up to 75% of infants with moderate to severe respiratory syncytial virus (RSV) bronchiolitis and is associated with significant morbidity and mortality in infants with congenital heart disease. The purpose of this study was to establish a mouse model of PH secondary to RSV bronchiolitis that mimics the disease etiology as it occurs in infants. Neonatal mice were infected with RSV at 5 days of age, and then re-infected 4 weeks later. Serum free medium was administered to age-matched mice as control. Echocardiography and right ventricular systolic pressure (RVSP) measurement via right jugular vein catheterization were conducted 5 and 6 days after the second infection, respectively. Peripheral capillary oxygen saturation (SpO2) monitoring did not indicate hypoxia at 2-4 days post-RSV infection, before reinfection, and at 2-7 days after reinfection. RSV infected mice had a significantly higher RVSP than the controls. Pulsed wave Doppler recording of the pulmonary blood flow by echocardiogram demonstrated significantly shortened pulmonary artery acceleration time (PAAT) and decreased PAAT/ejection time ratio in RSV infected mice. Morphom...
Source: American Journal of Physiology. Heart and Circulatory Physiology - Category: Physiology Authors: Tags: Am J Physiol Heart Circ Physiol Source Type: research