Down-regulation of CatSper 1 and CatSper 2 genes by lead and mercury

Publication date: Available online 8 March 2018 Source:Environmental Toxicology and Pharmacology Author(s): Sh Mohammadi, M Gholamin, M. Mohammadi, A. Mansouri, R. Mahmoodian, S. Attari, S.M. Kebriaei, B. Zibaei, M. Roshanaei, F. Daneshvar, M. Khandehro, M.A. Khodadadegan, A. Delshad, F. Mohammadzadeh, Mostafa Peyvandi, Majid Ghayour-Mobarhan, Shima Tavallaie, Samaneh Boroumand-Noughabi, Gordon A.A. Ferns In the study of the expression of CatSper genes, consideration of the effects of environmental metal toxicity is very important. Therefore, in this study, the effects of lead acetate and mercury chloride exposure on expression of CatSper genes, sperm parameters, histology of testis and prooxidant antioxidant balance (PAB) values of serum were investigated. A total of 28 mice was divided into four groups. The control group did not receive injections. The sham group received normal saline intraperitoneally. Lead and mercury groups were injected 60 and 1.25 mg/kg/daily lead acetate and mercury chloride respectively intraperitoneally for 2 weeks. After 35 days, the sperm analysis and histology of left testis were performed. In addition, serum was obtained to measure the PAB values. The right testis was used for molecular analysis of real-time PCR. Administration with either lead acetate or mercury caused significant damage to the seminiferous tubules as well as a reduction in sperm parameters compared to the control group. The relative expression of CatSper ...
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research