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Noggin Along with a Self-Assembling Peptide Nanofiber Containing Long Motif of Laminin Induces Tyrosine Hydroxylase Gene Expression

AbstractTyrosine hydroxylase (TH), a rate-limiting step in catecholamine synthesis in which its activity influences Alzheimer disease, Parkinson disease, and IQ of schizophrenia patients, has been studied for a long time. In the meantime, the present investigation assessed the effect of noggin and type of self-assembling nanofibers in TH gene over-expression by neuron-like cells derived from human endometrial-derived stromal cells (hEnSCs). Neuroblastoma cells and hEnSCs encapsulated into nanofibers including Matrigel, (RADA)4, laminin, and BMHP-1 motif bounded to (RADA)4 and their cell viability were studied for 48  h and 18 days in basal and neurogenic media, respectively, in noggin-rich media. Then, expression of neural genes and proteins has been investigated by immunocytochemistry (ICC) and real-time PCR methods, respectively. The results indicated that neuroblastoma cell and hEnSC viability is in good a greement with the level of Bcl2 and β-tubulin III gene expression; however, -BMHP-1 and -laminin nanofibers exhibited significantly higher cell viability eventually through Wnt/β-catenin signaling pathway as compared to others, respectively. The gene expression analysis of nanofibers showed that n one of them induced gamma-aminobutyric acid (GABA) gene expression while glial fibrillary acidic protein (GFAP) gene just over-expressed in cells encapsulated into Matrigel with a low level of Bcl2 gene expression. However, the TH gene just had been over-exp...
Source: Molecular Neurobiology - Category: Neurology Source Type: research

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In this study, we asked how POD is influenced by different experimental practices and how likely it is that underpowered experiments lead to scientific disputes between two groups conducting identical experiments. To address these questions, we generated a parametric model based on the Gompertz equation using lifespan data of 5,026 C. elegans. We then used this model to simulate lifespan experiments with different conditions to determine how experimental parameters affect the ability to detect lifespan increases of certain sizes. We considered two important experimental features that contribute to the workload of lifespan ...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
CONCLUSIONS: The present findings may be added to accumulated evidence on macroscopic and microscopic pathology of the cerebellum in schizophrenia. Morphological alterations of Purkinje cells seem to be a central feature of neuropathology of schizophrenia, reflecting to impairment of neuronal connectivity and functionality, and related to motor and cognitive symptoms. PMID: 28730225 [PubMed - in process]
Source: Romanian Journal of Morphology and Embryology - Category: General Medicine Tags: Rom J Morphol Embryol Source Type: research
Contributors : Dominique Arion ; John P Corradi ; Shaowu Tang ; Dibyadeep Datta ; Franklyn Boothe ; Aiqing He ; Angela M Cacace ; Robert Zaczek ; Charles F Albright ; George Tseng ; David A LewisSeries Type : Expression profiling by arrayOrganism : Homo sapiensSchizophrenia is associated with alterations in working memory that reflect dysfunction of dorsolateral prefrontal cortex (DLPFC) circuitry. Working memory depends on the activity of excitatory pyramidal cells in DLPFC layer 3, and to a lesser extent in layer 5.Although many studies have profiled gene expression in DLPFC gray matter in schizophrenia, little is known ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
CONCLUSIONS: We describe symptomatology, diagnostic and therapeutic options of gastric volvulus. PMID: 28728516 [PubMed - as supplied by publisher]
Source: Acta Chirurgica Belgica - Category: Surgery Tags: Acta Chir Belg Source Type: research
Dear Carol: My mom loves to shop, attend church, see local theater, and go to park events. We’ve done these things together for years. What’s changed is that Mom had a stroke last year. She recovered well, but she is unsteady on her feet when conditions aren’t perfect. She’s supposed to use a walker for balance, at least when she goes out of the house, but she refuses. I’m always nervous that she will fall, so I want her to hold onto me, but she hates that. I’ve begged and I’ve nagged, but I don’t get anywhere. How can I convince my stubborn mother to cooperate? – ...
Source: Minding Our Elders - Category: Geriatrics Authors: Source Type: blogs
Publication date: Available online 23 July 2017 Source:Molecular and Cellular Neuroscience Author(s): Nagaraj S. Moily, Angelique R. Ormsby, Aleksandar Stojilovic, Yasmin M. Ramdzan, Jeannine Diesch, Ross D. Hannan, Michelle S. Zajac, Anthony J. Hannan, Alicia Oshlack, Danny M. Hatters Huntington's disease is caused by polyglutamine (polyQ)-expansion mutations in the CAG tandem repeat of the Huntingtin gene. The central feature of Huntington's disease pathology is the aggregation of mutant Huntingtin (Htt) protein into micrometer-sized inclusion bodies. Soluble mutant Htt states are most proteotoxic and trigger an enhance...
Source: Molecular and Cellular Neuroscience - Category: Neuroscience Source Type: research
Discussion Health status limitations were associated with residential and, particularly, employment status independent from other, previously established predictors of everyday outcomes, including cognition and symptoms. The importance of health status limitations for predicting outcome was confirmed in both SCZ and BP, with schizophrenia representing the more impaired group.
Source: Journal of Psychiatric Research - Category: Psychiatry Source Type: research
Conclusions The study prioritizes significant DEGs commonly found in multiple datasets, through functional and protein interaction network analyses. The study also brings out the modulatory effects of eQTLs on DEGs and proposes novel therapeutic targets.
Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research
Source: Schizophrenia Research - Category: Psychiatry Source Type: research
CONCLUSIONS: Based on these naturalistic data, low exposure to adjunctive benzodiazepine and antidepressant medications does not seem to affect cognition nor explain the possible negative effects of high dose long-term antipsychotic medication on cognition in schizophrenia. PMID: 28728095 [PubMed - as supplied by publisher]
Source: Journal of the Association of European Psychiatrists - Category: Psychiatry Tags: Eur Psychiatry Source Type: research
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