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Noggin Along with a Self-Assembling Peptide Nanofiber Containing Long Motif of Laminin Induces Tyrosine Hydroxylase Gene Expression

AbstractTyrosine hydroxylase (TH), a rate-limiting step in catecholamine synthesis in which its activity influences Alzheimer disease, Parkinson disease, and IQ of schizophrenia patients, has been studied for a long time. In the meantime, the present investigation assessed the effect of noggin and type of self-assembling nanofibers in TH gene over-expression by neuron-like cells derived from human endometrial-derived stromal cells (hEnSCs). Neuroblastoma cells and hEnSCs encapsulated into nanofibers including Matrigel, (RADA)4, laminin, and BMHP-1 motif bounded to (RADA)4 and their cell viability were studied for 48  h and 18 days in basal and neurogenic media, respectively, in noggin-rich media. Then, expression of neural genes and proteins has been investigated by immunocytochemistry (ICC) and real-time PCR methods, respectively. The results indicated that neuroblastoma cell and hEnSC viability is in good a greement with the level of Bcl2 and β-tubulin III gene expression; however, -BMHP-1 and -laminin nanofibers exhibited significantly higher cell viability eventually through Wnt/β-catenin signaling pathway as compared to others, respectively. The gene expression analysis of nanofibers showed that n one of them induced gamma-aminobutyric acid (GABA) gene expression while glial fibrillary acidic protein (GFAP) gene just over-expressed in cells encapsulated into Matrigel with a low level of Bcl2 gene expression. However, the TH gene just had been over-exp...
Source: Molecular Neurobiology - Category: Neurology Source Type: research

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CONCLUSIONS: We found that acupuncture might be a safe and useful adjunctive treatment for patients with PD. However, because of methodological flaws in the included studies, conclusive evidence is still lacking. More rigorous and well-designed placebo-controlled trials should be conducted. PMID: 28917379 [PubMed - in process]
Source: Complementary Therapies in Medicine - Category: Complementary Medicine Authors: Tags: Complement Ther Med Source Type: research
CONCLUSION: Well-structured dance classes can promote social-connectedness and joy, in addition to facilitating movement to music and physical activity. Consumers advised that careful planning of the classes and tailoring them to participant needs optimizes outcomes. PMID: 28917359 [PubMed - in process]
Source: Complementary Therapies in Medicine - Category: Complementary Medicine Authors: Tags: Complement Ther Med Source Type: research
OBJECTIVE: To summarise the available literature on driving with Alzheimer's disease (AD) and to investigate the relationship between driving and cognitive functioning. DESIGN: Literature review. METHOD: A systematic search of the electroni...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Distraction, Fatigue, Chronobiology, Vigilance, Workload Source Type: news
Authors: Gaszner G, Barna G, Dome P Abstract The history of antipsychotics began with the discovery of chlorpromazine in the 1950s. Since then this group of medications has become one of the most important element of the armamentarium of psychopharmacology. While initially these pharmacons were used in the treatment of psychotic states (including psychotic mania) in the last approximately 10-15 years new indications - such as treatment of depressive, manic and mixed states and also mood-stabilization in bipolar disorder and also the treatment of major depressive disorder - for several second-generation antipsychoti...
Source: Neuropsychopharmacologia Hungarica - Category: Psychiatry Tags: Neuropsychopharmacol Hung Source Type: research
CONCLUSIONS: Early onset of the disease, bipolar I diagnosis and comorbid personality disorder were typical of psychotic bipolar patients; in addition, suicide attempts and residual symptoms were more common compared to the non-psychotic group. LIMITATIONS: The relatively lower number of patients (89 and 80 patients) and the fact that the data were processed retrospectively limit the generalizability of our results. PMID: 28918417 [PubMed - in process]
Source: Neuropsychopharmacologia Hungarica - Category: Psychiatry Tags: Neuropsychopharmacol Hung Source Type: research
Xiaoliang Li, YaNan Zhang, Yu Wang, Jing Xu, Ping Xin, YongHai Meng, Qiuhong Wang, Haixue Kuang
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
This article reviews three different computational approaches and their possible complementarities. Process algebras, implemented using declarative programming languages such as Maude, facilitate simulation and analysis of complicated biological processes on a comprehensive but coarse-grained level. A model-driven Integration of Data and Knowledge, based on the OpenBEL platform and using reverse causative reasoning and network jump analysis, can generate mechanistic knowledge and a new, mechanism-based taxonomy of disease. Finally, Quantitative Systems Pharmacology is based on formalized implementation of domain expertise ...
Source: The Journal of Alzheimers Association - Category: Psychiatry Tags: Alzheimers Dement Source Type: research
AbstractThe prognosis for children with high-risk neuroblastoma is often poor and survivors can suffer from severe side effects. Predictive preclinical models and novel therapeutic strategies for high-risk disease are therefore a clinical imperative. However, conventional cancer cell line-derived xenografts can deviate substantially from patient tumors in terms of their molecular and phenotypic features. Patient-derived xenografts (PDXs) recapitulate many biologically and clinically relevant features of human cancers. Importantly, PDXs can closely parallel clinical features and outcome and serve as excellent models for bio...
Source: Cell and Tissue Research - Category: Cytology Source Type: research
This study investigated the influence of BDNF deficiency and NMDA receptor hypofunction on electrophysiological responses to brief acoustic stimuli. Adult BDNF heterozygote (BDNF+/‐) and wildtype littermate C57Bl/6J mice were surgically implanted with EEG recording electrodes. All mice underwent EEG recording sessions to measure ongoing and auditory‐evoked electrophysiological responses following treatment with MK‐801 (0.3mg/kg ip) or vehicle. Western blotting on post‐mortem cortical tissue assessed parvalbumin and GAD67 expression – markers of interneurons which are involved in the generation of gamma oscill...
Source: European Journal of Neuroscience - Category: Neuroscience Authors: Tags: Special Issue Article Source Type: research
Filippo S. Giorgi, Larisa Ryskalin, Riccardo Ruffoli, Francesca Biagioni, Fiona Limanaqi, Michela Ferrucci, Carla L. Busceti, Ubaldo Bonuccelli, Francesco Fornai
Source: Frontiers in Neuroanatomy - Category: Neurology Source Type: research
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