UCA1 Regulates the Growth and Metastasis of Pancreatic Cancer By Sponging MiR-135a.

This study is aimed to investigate the role of urothelial carcinoma associated 1(UCA1) in the progression of PC. Our results revealed that long non-coding RNAs (lncRNAs)-UCA1 was overexpressed in PC tissues compared with adjacent histologically normal tissues. Down-regulated level of UCA1 was also detected in five human pancreatic cancer cell lines (SW1990，BxPC-3, MiaPaCa-2, PANC-1, CAPAN-1) compared with normal pancreatic duct epithelial HPDE cells. The proliferation of PC cells was inhibited after UCA1 was suppressed by a lentiviral vector. Cell apoptosis rate was largely promoted by down-regulating UCA1. Further research revealed that microRNA (miRNA)-135a is a direct target of UCA1. The expression of miR-135a was decreased in PC tissues and cell lines compared with control groups. Besides, the decreased level of miR-135a was elevated adding miR-135a mimic in SW1990 cells transfected with LncRNA-UCA1. Similarly, up-regulated level of miR-135a was down-regulated adding miR-135a inhibitor in SW1990 cells transfected with UCA1 siRNA. Luciferase activity assay further comfirmed the targeting relationship between UCA1 and miR-135a. Moreover, miRNA-135a reversed the effect of UCA1 on cell apoptosis rate and cell viability in SW1990 cells. The migration and invasion capacity of PC cells suppressed by UCA1 siRNA was then enhanced by miR-135a inhibitor. <i>In vivo</i>, UCA1 siRNA effectively suppressed the tumor growth and the expression of migration markers. Taken to...
Source: Oncology Research - Category: Cancer & Oncology Tags: Oncol Res Source Type: research