Abstract B07: Tead and AP1 coordinate transcription and motility in cancer cells

The Tead family transcription factors are the major mediators of the Hippo-Yap pathway. Although misregulation of Hippo signaling has been implicated in many human cancers, Tead dependent downstream transcriptional programs in cancer cells remain poorly understood. Here we characterize Tead4-mediated transcriptional networks in diverse cancer cells, including neuroblastoma, colorectal, lung, and endometrial carcinomas. By intersecting genome-wide chromatin occupancy analyses of Tead4, JunD, and Fra1/2, we find that Tead4 cooperates with AP1 factors to coordinate target gene transcription. We find that the interaction of AP1 and Tead4 is JNK independent, but engages the SRC coactivators to promote downstream transcription. Furthermore we show that Tead and AP1 drive the expression of a unique core set of target genes to direct cell motility and invasion. Together, our data unveil a critical regulatory mechanism coordinating AP1 and Tead -controlled transcriptional and functional output in cancer cells.Citation Format: Xiangfan Liu, Huapeng Li, Mihir Rajurkar, Jennifer Cotton, Arthur Mercurio, Roger Davis, Junhao Mao. Tead and AP1 coordinate transcription and motility in cancer cells. [abstract]. In: Proceedings of the AACR Special Conference: Developmental Biology and Cancer; Nov 30-Dec 3, 2015; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Res 2016;14(4_Suppl):Abstract nr B07.
Source: Molecular Cancer Research - Category: Cancer & Oncology Authors: Tags: Signaling Pathways: Hippo/TOR: Poster Presentations - Proffered Abstracts Source Type: research