The effect of fetal rat brain extract on morphology of bone marrow-derived mesenchymal stem cells

Abstract Nowadays, regenerative medicine is going to find its true position among different kinds of treatment measures. In this regard, cell therapy brings hopes for the treatment of various diseases. As it is obvious, the role of nervous system for the appropriate function of other organs is not negligible. Unfortunately, damage to nerve cells is irreversible. Substitution of malfunctioned neural cells with the normal one is a noteworthy choice. The aim of the present study was to determine the effect of fetal rat brain extract on morphology of bone marrow-derived mesenchymal stem cells (BM-MSCs). Hence, pooled BM-MSCs were obtained from femurs and tibias of rats and cultured until reaching passage 3. BM-MSCs were characterized by the presence of mesenchymal specific stem cell markers and the absence of hematopoietic specific stem cell markers. Also, to confirm the BM-MSC potency, osteogenic and adipogenic differentiation capability was done. Fetal brain extract were prepared from rat in the late second week of gestational period. BM-MSCs were treated with basal culture medium containing 20 % brain extract. Control group was only treated with basal medium. After 3 days, neural-like cells were noticed with round body and significant projections. In some cases, the cells were bipolar and in the remained were multipolar. Cells with such structures were not seen in the control group. As the brain extract is readily available, inexpensive, do not cause any tox...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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This article is part of a Special Issue entitled: Cardiomyocyte biology: new pathways of differentiation and regeneration edited by Marijke Brink, Marcus C. Schaub, and Christian Zuppinger.
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - Category: Molecular Biology Source Type: research
Last year, Molecular Brain became the first BMC journal to introduce Micro Reports, an innovative way to quickly share research which would otherwise go unpublished as a full research paper. Micro Reports are short research summaries of up to 1200 words, ideal for publishing negative results, reproduced studies, smaller data sets which are still important but would not warrant a full research article, and brand new findings which should enter the public domain as soon as possible. Since the launch of this new article type, the journal has received 58 Micro Report submissions, with 24 publications to date. We’ve highl...
Source: BioMed Central Blog - Category: General Medicine Authors: Tags: Biology Open Access Publishing Molecular Brain neuroscience SfN19 Source Type: blogs
Schematic overview of current hiPSCs disease 3D retinal modeling. Somatic cells from patients are reprogrammed toward human induced pluripotent stem cells (hiPSCs). Derived 3D retinal organoids from hiPSCs serve as a model for further investigation of disease mechanisms, drug, and toxicological screening as well as for future developments of new therapies in patients. The application of gene ‐editing technology in patient's hiPSCs could create the gene‐corrected 3D organoids as a cell source for transplantation therapy of hereditary retinal dystrophies. Abbreviations: PRs, photoreceptors; GCs, ganglion cells; HCs, hori...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research
Approximately one in every 50-100 people is affected with bipolar disorder (BD), making this disease a major economic burden. The introduction of the induced pluripotent stem cells (iPSCs) methodology enabled better modeling of this disorder.
Source: Biological Psychiatry - Category: Psychiatry Authors: Tags: Archival Report Source Type: research
Abstract A functional bipolar spindle is essential to segregate chromosomes correctly during mitosis. Across organisms and cell types, spindle architecture should be optimized to promote error-free divisions. However, it remains to be investigated whether mitotic spindle morphology adapts to changes in tissue properties, typical of embryonic development, in order to ensure different tasks, such as spindle positioning and chromosome segregation. We have characterized mitotic spindles in neural stem cells (NSCs) of the embryonic developing mouse neocortex. Surprisingly, we found a switch in spindle morphology f...
Source: Current Biology - Category: Biology Authors: Tags: Curr Biol Source Type: research
The nucleosome remodeling and deacetylase (NuRD) complex presents one of the major chromatin remodeling complexes in mammalian cells. Here we discuss current evidence for NuRD’s role as an important epigenetic regulator of gene expression in neural stem cell (NSC) and neural progenitor cell (NPC) fate decisions in brain development. With the formation of the cerebellar and cerebral cortex, NuRD facilitates experience-dependent cerebellar plasticity and regulates additionally cerebral subtype specification and connectivity in postmitotic neurons. Consistent with these properties, genetic variation in NuRD’s subu...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
Transposable elements (TEs) are highly repetitive DNA sequences in the human genome that are the relics of previous retrotransposition events. Although the majority of TEs are transcriptionally inactive due to acquired mutations or epigenetic processes, around 8% of TEs exert transcriptional activity. It has been found that TEs contribute to somatic mosaicism that accounts for functional specification of various brain cells. Indeed, autonomous retrotransposition of long interspersed element-1 (LINE-1) sequences has been reported in the neural rat progenitor cells from the hippocampus, the human foetal brain and the human e...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
Abstract Dopaminergic (DA) neurons are involved in many critical functions within the central nervous system (CNS), and dopamine neurotransmission impairment underlies a wide range of disorders from motor control deficiencies, such as Parkinson's disease (PD), to psychiatric disorders, such as alcoholism, drug addictions, bipolar disorders, schizophrenia and depression. Neural stem cell-based technology has potential to play an important role in developing efficacious biological and small molecule therapeutic products for disorders with dopamine dysregulation. Various methods of differentiating DA neuron...
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research
In this study, the anti-cancer effects of different anti-psychotic medicines on various malignant tumor cells and their molecular mechanisms have been discussed. PMID: 31108054 [PubMed - as supplied by publisher]
Source: European Journal of Pharmacology - Category: Drugs & Pharmacology Authors: Tags: Eur J Pharmacol Source Type: research
We reported DEGs with a nominal p-value of 0.8. The network construction was created using blockwiseModules function for consideration of computer efficiency. The module detection criteria were as follows: minimum module size of 50, deepsplit of 4, and merge threshold of 0.25. The merged modules were then summarized with module eigengene (ME) correlations>0.75. MEs were defined by their first principal component and were labeled with different colors as module names in the Results section. After the modules were generated, we conducted different enrichment analysis to explore the functional interpretation of genes withi...
Source: Frontiers in Psychiatry - Category: Psychiatry Source Type: research
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