Technologies for the discovery of G protein-coupled receptor-targeting biologics

Curr Opin Biotechnol. 2024 May 9;87:103138. doi: 10.1016/j.copbio.2024.103138. Online ahead of print.ABSTRACTG protein-coupled receptors (GPCRs) are important pharmaceutical targets, working as entry points for signaling pathways involved in metabolic, neurological, and cardiovascular diseases. Although small molecules remain the major GPCR drug type, biologic therapeutics, such as peptides and antibodies, are increasingly found among clinical trials and Food and Drug Administration (FDA)-approved drugs. Here, we review state-of-the-art technologies for the engineering of biologics that target GPCRs, as well as proof-of-principle technologies that are ripe for this application. Looking ahead, inexpensive DNA synthesis will enable the routine generation of computationally predesigned libraries for use in display assays for the rapid discovery of GPCR binders. Advances in synthetic biology are enabling the increased throughput of functional GPCR assays to the point that they can be used to directly identify biologics that modulate GPCR activity. Finally, we give an overview of adjacent technologies that are ripe for application to discover biologics that target human GPCRs.PMID:38728825 | DOI:10.1016/j.copbio.2024.103138
Source: Current Opinion in Biotechnology - Category: Biotechnology Authors: Source Type: research