WITHANICANDRIN ISOLATED FROM Datura Ferox PROMOTES ANTINOCICEPTION BY MODULATING THE ASICS AND TRPS CHANNELS AND ANTI-INFLAMMATION IN ADULT ZEBRAFISH

Chem Biodivers. 2024 Apr 19:e202400538. doi: 10.1002/cbdv.202400538. Online ahead of print.ABSTRACTThis is the first study to analyze the anti-inflammatory and antinociceptive effect of withanicandrin, isolated from Datura Ferox leaves, and the possible mechanism of action involved in adult zebrafish (ZFa). To this end, the animals were treated intraperitoneally (i.p.) with withanicandrin (4; 20 and 40 mg/kg; 20 μL) and subjected to locomotor activity and acute toxicity. Nociception tests were also carried out with chemical agents, in addition to tests to evaluate inflammatory processes induced by κ-Carrageenan 1.5% and a Molecular Docking study. As a result, withanicandrin reduced nociceptive behavior by capsaicin at a dose of 40 mg/kg and by acid saline at doses of 4 and 40 mg/kg, through neuromodulation of TRPV1 channels and ASICs, identified through blocking the antinociceptive effect of withanicandrin by the antagonists capsazepine and naloxone. Furthermore, withanicandrin caused an anti-inflammatory effect through the reduction of abdominal edema, absence of leukocyte infiltrate in the liver tissue and reduction of ROS in thel liver tissue and presented better affinity energy compared to control morphine (TRPV1) and ibuprofen (COX-1 and COX-2).PMID:38639566 | DOI:10.1002/cbdv.202400538

Source: Chemistry and Biodiversity - April 19, 2024 Category: Biochemistry Authors: J éssica Bezerra Maciel Hort ência Ribeiro Liberato Ant ônio Wlisses da Silva Jo ão Pedro Vieira da Silva Francisco das Chagas L Pinto Emanuela de Lima Rebou ças Francisco Sydney Henrique da Silva Maria Kuerislene Am âncio Ferreira Marcia Machado Ma Source Type: research