Circ_100549 promotes tumor progression in lung adenocarcinoma through upregulation of BIRC6

AbstractLung adenocarcinoma (LUAD) is a subtype of lung cancer with high incidence and mortality globally. Emerging evidence suggests that circular RNAs (circRNAs) exert critical functions in human cancers, including LUAD.CircRNA_100549 (circ_100549) has been reported to be significantly upregulated in non-small cell lung cancer (NSCLC) samples, while its role in modulating LUAD progression remains to be explored. The current study aims at investigating the functional roles ofcirc_100549 in LUAD and its downstream molecular mechanism. First, we found that the expression ofcirc_100549 was higher in LUAD cell lines. Loss-of-function assays verified that depletion ofcirc_100549 repressed LUAD cell proliferation but accelerated cell apoptosis. Furthermore, in vivo experiments demonstrated that silencing ofcirc_100549 suppressed tumor growth. Subsequently, based on database analysis, we carried out a series of experiments to explore the mechanisms and effects ofcirc_100549 underlying LUAD progression, including RNA-binding protein immunoprecipitation (RIP), RNA/DNA pull-down, luciferase reporter, and chromatin immunoprecipitation (ChIP) assays. The results indicated thatcirc_100549 serves as a ceRNA by spongingmiR-95-5p to upregulateBPTF expression, thus upregulatingBIRC6 expression at a transcriptional level in LUAD. In summary, our study demonstrated thatcirc_100549 facilitates LUAD progression by upregulatingBIRC6 expression.
Source: Histochemistry and Cell Biology - Category: Biomedical Science Source Type: research