Single Cell Transcriptomics of Mouse Epidermis Reveals a Pivotal Role of Langerhans Cells in Painful Diabetic Neuropathy

Painful diabetic neuropathy (PDN) is one of the most common and intractable complications of diabetes, resulting in remodeling of the cutaneous innervation and neuropathic pain. Accumulating evidence suggests the crucial role epidermal non-neuronal cells for the development of PDN. To understand how epidermal cells communicate with cutaneous afferents and how this communication affects PDN, we performed single-cell RNA sequencing of the epidermis of mice fed a high-fat diet (HFD) for 10 weeks. We captured the gene expression profile of keratinocytes and non-keratinocytes cells, including Langerhans cells (LCs).
Source: The Journal of Pain - Category: Materials Science Authors: Source Type: research