Viruses, Vol. 16, Pages 548: Pandemic Risk Assessment for Swine Influenza A Virus in Comparative In Vitro and In Vivo Models

Viruses, Vol. 16, Pages 548: Pandemic Risk Assessment for Swine Influenza A Virus in Comparative In Vitro and In Vivo Models Viruses doi: 10.3390/v16040548 Authors: Ian Padykula Lambodhar Damodaran Kelsey T. Young Madelyn Krunkosky Emily F. Griffin James F. North Peter J. Neasham Vasilis C. Pliasas Chris L. Siepker James B. Stanton Elizabeth W. Howerth Justin Bahl Constantinos S. Kyriakis Stephen Mark Tompkins Swine influenza A viruses pose a public health concern as novel and circulating strains occasionally spill over into human hosts, with the potential to cause disease. Crucial to preempting these events is the use of a threat assessment framework for human populations. However, established guidelines do not specify which animal models or in vitro substrates should be used. We completed an assessment of a contemporary swine influenza isolate, A/swine/GA/A27480/2019 (H1N2), using animal models and human cell substrates. Infection studies in vivo revealed high replicative ability and a pathogenic phenotype in the swine host, with replication corresponding to a complementary study performed in swine primary respiratory epithelial cells. However, replication was limited in human primary cell substrates. This contrasted with our findings in the Calu-3 cell line, which demonstrated a replication profile on par with the 2009 pandemic H1N1 virus. These data suggest that the selection of models is important for meaningful risk assessment.
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research