3D Object Geometry of Mitochondria-Associated Signal: 3D Analysis Pipeline for Two-Photon Image Stacks of Cerebrovascular Endothelial Mitochondria

Am J Physiol Heart Circ Physiol. 2024 Mar 22. doi: 10.1152/ajpheart.00101.2024. Online ahead of print.ABSTRACTIncreasing evidence indicates the role of mitochondrial and vascular dysfunction in aging and aging-associated pathologies; however, the exact mechanisms and chronological processes remain enigmatic. High energy demand organs, such as the brain, depend on the health of their mitochondria and vasculature in the maintenance of normal functions, therefore representing vulnerable targets for aging. This methodology paper describes an analysis pipeline for 3D mitochondria-associated signal geometry of two-photon image stacks of brain vasculature. The analysis methods allow the quantification of mitochondria-associated signals obtained in real time in their physiological environment. In addition, signal geometry results will allow the extrapolation of fission and fusion events under normal conditions, during aging, or in the presence of different pathological conditions; therefore, contributing to our understanding of the role mitochondria play in a variety of aging-associated diseases with vascular etiology.PMID:38517228 | DOI:10.1152/ajpheart.00101.2024
Source: American Journal of Physiology. Heart and Circulatory Physiology - Category: Physiology Authors: Source Type: research