Inhibitory protein –protein interactions of the SIRT1 deacetylase are choreographed by post‐translational modification

We present the x-ray structure of the DBC1 S1-like domain that binds SIRT1 and an NMR characterization of how the SIRT1 N-terminal region engages DBC1. This interaction is inhibited by acetylation of K112 of DBC1 and stimulated by the insulin-dependent phosphorylation of human SIRT1 at S162 and S172, catalyzed sequentially by CK2 and GSK3, resulting in the PACS-2-dependent inhibition of nuclear SIRT1 enzymatic activity and translocation of the deacetylase in the cytoplasm. Finally, we discuss how defects in the DBC1/PACS-2-controlled SIRT1 inhibitory pathway are associated with disease, including obesity and non-alcoholic fatty liver disease.

Source: Protein Science - March 27, 2024 Category: Biochemistry Authors: Troy C. Krzysiak, You ‐Jin Choi, Yong Joon Kim, Yunhan Yang, Christopher DeHaven, Lariah Thompson, Ryan Ponticelli, Mara M. Mermigos, Laurel Thomas, Andrea Marquez, Ian Sipula, Jongsook Kim Kemper, Michael Jurczak, Gary Thomas, Angela M. G Tags: RESEARCH ARTICLE Source Type: research