Human Amniotic MSC Response in LPS-Stimulated Ascites from Patients with Cirrhosis: FOXO1 Gene and Th17 Activation in Enhanced Antibacterial Activation

In this study, we used an in vitro SBP model and employed omics techniques (next-generation sequencing) to investigate the mechanisms by which hA-MSCs modify the crosstalk between immune cells in LPS-stimulated ascitic fluid. We also validated the data obtained via qRT-PCR, cytofluorimetric analysis, and Luminex assay. These findings provide further support to the hope of using hA-MSCs for the prevention and treatment of infective diseases, such as SBP, offering a viable alternative to antibiotic therapy.PMID:38474048 | DOI:10.3390/ijms25052801

Source: Molecular Medicine - March 13, 2024 Category: Molecular Biology Authors: Mariangela Pampalone Nicola Cuscino Gioacchin Iannolo Giandomenico Amico Camillo Ricordi Giampiero Vitale Claudia Carcione Salvatore Castelbuono Simone Dario Scilabra Claudia Coronnello Salvatore Gruttadauria Giada Pietrosi Source Type: research