Protective effect of human epicardial adipose ‐derived stem cells on myocardial injury driven by poly‐lactic acid nanopillar array

AbstractWe investigated if poly-lactic acid (PLA) nanopillar array can trigger the differentiation of human epicardial (ADSCs) (heADSCs) into cardiomyocyte-like cells and explored the effects of these cardiomyocyte-like cells on myocardial infarction (MI) in vivo. PLA nanopillar array (200  nm diameter) and plain PLA film (PLA planar) induced heADSCs were marked with carboxyfluorescein. After 7 days, the expressions of myocardiocyte-specific genes were significantly enhanced in cells seeded on PLA nanopillar array compared with that on PLA planar, especiallyCACNA1C,KCNH2, andMYL2 genes (p <  0.05). However, the expressions of cardiac troponin T(cTNT),KCNQ1, andKCNA5 were lower than those in PLA planar-induced heADSCs (p <  0.05), whereasGATA4 tended to increase with time. The cells with positively stainedα-actinin andcTNT were elevated in heADSCs induced by PLA nanopillar array compared with those induced by PLA planar only (p <  0.05). In vivo experiments showed that cardiac function was improved after injecting PLA-nanopillar array-induced heADSCs into the ischemic heart (p <  0.05, compared with PLA planar + MI group). Furthermore, tyrosine hydroxylase density was significantly lower (p <  0.05). PLA nanopillar array directly drives the differentiation of heADSCs into cardiomyocyte-like cells, and the induced heADSCs exhibit a protective effect on ischemic myocardium by improving cardiac function in MI rats.
Source: Biotechnology and Applied Biochemistry - Category: Biochemistry Authors: Tags: ORIGINAL ARTICLE Source Type: research