Genetic diversity and haplotypes of Echinococcus granulosus isolated from cattle and buffaloes and first report of E. ortleppi (G5) in buffaloes in Pakistan based on mitochondrial cytochrome c oxidase subunit-1 gene (mt-CO1) markers

Exp Parasitol. 2023 Nov 7:108648. doi: 10.1016/j.exppara.2023.108648. Online ahead of print.ABSTRACTCystic echinococcosis (CE) is a parasitic disease, caused by larval stage of Echinococcus granulosus sensu lato, one of the most important and neglected zoonotic diseases. Although the disease is endemic in neighboring countries, information regarding genetic diversity of E. granulosus s.l. is scarce in Pakistan. Therefore, the main purpose of this report was to contribute in molecular epidemiology and to find genetic diversity and haplotypes of E. granulosus s.l. in cattle and buffalo isolates. To identify species circulating in country, parasite materials were collected from different slaughterhouses and butcher shops of two major cities, Rawalpindi and Peshawar located in Punjab and Khyber Pakhtunkhwa (KP) provinces, Pakistan, respectively. A total of 100 CE cyst samples were investigated from buffalo (n = 61), cattle (n = 39) hosts. After genomic DNA extraction from individual cyst materials, mt-CO1 (875 bp) gene was amplified by PCR. After that, PCR products were electrophoresed on the agarose gel then purified and sequenced using forward primer. The sequences were trimmed (779 bp), aligned and compared using published sequences in NCBI. E. granulosus s.s. (G1, G3) (71.4%; n = 20/28) was confirmed as the dominant species in buffalo and cattle. E. ortleppi (G5) (28.6%; n = 8/28) was recorded for the first time in both buffalo and cattle isolates from Rawalpindi. E. granulos...
Source: Experimental Parasitology - Category: Parasitology Authors: Source Type: research