Immunostimulant effects of diet supplementation with yellow (Pouteria campechiana), white (Casimiroa edulis), and black (Diospyros digyna) sapote nanocapsules on laying hens: in vitro and in vivo study

AbstractPoultry is commonly infected by different bacteria and parasites in the environment, resulting in increased morbidity and mortality, but immunostimulants have been enhancing non-specific defense mechanisms conferring laying hens ’ protection. For this purpose, the pulp of yellow (Pouteria campechiana), white (Casimiroa edulis), and black (Diospyros digyna) sapotes were nanoencapsulated (YWB-SN) and evaluated in laying hens ’ peripheral blood leukocytes to test their addition to the experimental diets at a concentration of 0.5% (5g/kg of dry food) for 1 month (with two samples at days 15 and 30). TheYWB-SN were safe when exposed to peripheral blood leukocytes (PBLs). Thein vitro experiment showed that these nanocapsules enhanced reactive oxygen species production, andB-SN stimulated phagocytosis activity. Concerning the proinflammatory cytokine (TNF- α) transcription, this gene was upregulated afterW-SN stimulation, whileB-SN upregulated the IgG gene expression significantly. IgM was upregulated with anyYBW-SN in PBLs after 24 h of stimulation. Thein vivo study showed a notableB-SN immunostimulation in serum and an upregulation of TNF- α, IgM, and IgG mRNA transcription. Therefore, this study provides a new result of the yellow, white, and black sapote nanocapsules as a functional food for the poultry industry, highlighting the black sapoteDiospyros digyna immunostimulant effect.
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research