Role of senkyunolide I in the promotion of neural stem/progenitor cell proliferation via the Akt/ β-catenin pathway

This study examined the effects of SEI on the proliferation and neuronal lineage differentiation of prepared neural stem/progenitor cells (NS/PCs). The NS/PC proliferation was determined by 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt, and neurosphere formation assays. The NS/PC differentiation was also investigated by immunocytochemistry, and western blotting was employed to measure phosphorylated Akt (pAkt) and GSK-3β (pGSK-3β), and active-β-catenin protein levels. We showed that the NS/PC proliferation was enhanced after SEI exposure. Elevated cell numbers were also observed in neurospheres, which were incubated with SEI for 3 days, whereas the NS/PC differentiation was decreased after SEI exposure for 5 days. Furthermore, SEI upregulated pAkt/Akt and active-β-catenin levels and increased NS/PC proliferation after SEI treatment was reversed by phosphatidylinositol 3-kinase inhibitor LY294002. downregulated differentiated processes. Thus, SEI promoted the NS/PC proliferation and suppressed NS/PC differentiation into neurons and/or astrocytes, therefore SEI could be an interesting and promising candidate for stimulating NSCs.PMID:37832402 | DOI:10.1016/j.biopha.2023.115683

Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - October 13, 2023 Category: Drugs & Pharmacology Authors: Min Wang Hideki Hayashi Ichiro Horinokita Mayumi Asada Yui Iwatani Jun-Guo Ren Jian-Xun Liu Norio Takagi Source Type: research